| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | cry1Bb |
| Uniprot No | Q45739 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1229aa |
| 氨基酸序列 | MTSNRKNENEIINALSIPTVSNPSTQMNLSPDARIEDSLCVAEVNNIDPFVSASTVQTGINIAGRILGVLGVPFAGQLASFYSFLVGELWPSGRDPWEIFLEHVEQLIRQQVTENTRNTAIARLEGLGRGYRSYQQALETWLDNRNDARSRSIILERYVALELDITTAIPLFRIRNEEVPLLMVYAQAANLHLLLLRDASLFGSEWGMASSDVNQYYQEQIRYTEEYSNHCVQWYNTGLNNLRGTNAESWLRYNQFRRDLTLGVLDLVALFPSYDTRTYPINTSAQLTREIYTDPIGRTNAPSGFASTNWFNNNAPSFSAIEAAIFRPPHLLDFPEQLTIYSASSRWSSTQHMNYWVGHRLNFRPIGGTLNTSTQGLTNNTSINPVTLQFTSRDVYRTESNAGTNILFTTPVNGVPWARFNFINPQNIYERGATTYSQPYQGVGIQLFDSETELPPETTERPNYESYSHRLSHIGLIIGNTLRAPVYSWTHRSADRTNTIGPNRITQIPLVKALNLHSGVTVVGGPGFTGGDILRRTNTGTFGDIRLNINVPLSQRYRVRIRYASTTDLQFFTRINGTTVNIGNFSRTMNRGDNLEYRSFRTAGFSTPFNFLNAQSTFTLGAQSFSNQEVYIDRVEFVPAEVTFEAEYDLERAQKAVNALFTSTNPRRLKTDVTDYHIDQVSNMVACLSDEFCLDEKRELFEKVKYAKRLSDERNLLQDPNFTFISGQLSFASIDGQSNFPSINELSEHGWWGSANVTIQEGNDVFKENYVTLPGTFNECYPNYLYQKIGESELKAYTRYQLRGYIEDSQDLEIYLIRYNAKHETLDVPGTDSLWPLSVESPIGRCGEPNRCAPHFEWNPDLDCSCRDGERCAHHSHHFTLDIDVGCTDLHENLGVWVVFKIKTQEGYARLGNLEFIEEKPLIGEALSRVKRAEKKWRDKREKLQLETKRVYTEAKEAVDALFVDSQYDQLQADTNIGMIHAADKLVHRIREAYLSELPVIPGVNAEIFEELEGHIITAMSLYDARNVVKNGDFNNGLTCWNVKGHVDVQQSHHRSDLVIPEWEAEVSQAVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFKNCEEEEVYPTDTGTCNDYTAHQGTAACNSRNAGYEDAYEVDTTASVNYKPTYEEETYTDVRRDNHCEYDRGYVNYPPVPAGYVTKELEYFPETDTVWIEIGETEGKFIVDSVELLLMEE |
| 预测分子量 | 139,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cry1Bb重组蛋白的3篇示例参考文献(内容为假设性示例，供参考)：
1. **标题**: "Expression and insecticidal activity of recombinant Cry1Bb protein in Bacillus thuringiensis"
**作者**: Zhang, Y., et al.
**摘要**: 研究通过基因重组技术在苏云金芽孢杆菌中高效表达Cry1Bb蛋白，并验证其对玉米螟幼虫的杀虫活性。结果显示，重组蛋白对目标害虫的半数致死浓度(LC50)显著低于天然蛋白，可能与蛋白质结构稳定性提升有关。
2. **标题**: "Structural analysis of Cry1Bb domain III and its role in receptor binding"
**作者**: Liu, H., & Wang, X.
**摘要**: 通过X射线晶体学解析Cry1Bb蛋白的结构，重点关注其结构域III与昆虫中肠受体蛋白的互作机制。实验表明，结构域III的特定氨基酸突变会显著降低其对亚洲玉米螟的毒性。
3. **标题**: "Development of Cry1Bb-resistant insect populations and cross-resistance studies"
**作者**: Gupta, R., et al.
**摘要**: 长期暴露于Cry1Bb蛋白的害虫种群产生抗性，研究发现抗性机制与中肠刷状缘膜囊泡(BBMV)中ALP酶表达下调相关。研究为抗性管理策略提供了依据。
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如需查找真实文献，建议在 **PubMed** 或 **Google Scholar** 中搜索关键词：
`Cry1Bb recombinant protein insecticidal activity` 或 `Cry1Bb structure function`。
The Cry1Bb recombinant protein is a genetically engineered variant derived from the naturally occurring Cry1B protein produced by the soil bacterium *Bacillus thuringiensis* (Bt). Bt is widely studied for its insecticidal properties, as it synthesizes crystalline (Cry) proteins during sporulation. These proteins are toxic to specific insect pests, particularly Lepidoptera (e.g., moths and butterflies), by binding to midgut receptors, disrupting membrane integrity, and causing cell lysis. Cry1Bb, a member of the Cry1 toxin family, shares structural similarities with other Cry proteins, featuring three conserved domains involved in receptor binding and pore formation.
Recombinant Cry1Bb is generated through molecular cloning, where the *cry1Bb* gene is inserted into expression vectors (e.g., *E. coli* or yeast systems) for large-scale production. This recombinant approach allows for enhanced protein yield, purity, and stability compared to native Bt extracts. Researchers have optimized Cry1Bb for broader or more potent insecticidal activity, often through domain swapping or mutagenesis to overcome insect resistance or expand target specificity. Its efficacy against pests like the European corn borer (*Ostrinia nubilalis*) and corn earworm (*Helicoverpa zea*) has made it a candidate for transgenic crops, such as Bt maize, to reduce chemical pesticide reliance.
However, Cry1Bb’s deployment raises ecological and regulatory considerations, including potential impacts on non-target organisms and the evolution of resistance in pest populations. Studies continue to assess its environmental persistence, toxicity profiles, and compatibility with integrated pest management strategies. As a recombinant protein, Cry1Bb exemplifies the intersection of biotechnology and sustainable agriculture, balancing pest control benefits with biosafety challenges.
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