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Mouse Monoclonal HIST2H4A(20Me3) Antibody

  • 中文名: HIST2H4A(20Me3)抗体
  • 别    名: H4; H4/n; H4F2; H4FN; FO108; HIST2H4
货号: IPD30889
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/200 - 1/400 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesH4; H4/n; H4F2; H4FN; FO108; HIST2H4
Entrez GeneID8370
clone7A2A7
WB Predicted band size11.4kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenSynthesized peptide of human HIST2H4A (AA: GGAKRHRK(Me3)VLRDNIQ) .
FormulationPurified antibody in PBS with 0.05% sodium azide

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参考文献

以下是3篇关于H4K20me3(可能与HIST2H4A相关)抗体的参考文献,基于组蛋白修饰H4K20三甲基化的研究:

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1. **文献名称**:*"Suv4-20h histone methyltransferases promote heterochromatin nuclear organization"*

**作者**:Schotta G, et al.

**摘要**:研究揭示了Suv4-20h1/h2甲基转移酶对H4K20me3的催化作用及其在异染色质结构维持中的关键功能,通过特异性抗体验证了H4K20me3在端粒和着丝粒区域的富集。

2. **文献名称**:*"Dynamic regulation of histone H3K9 and H4K20 tri-methylation in pluripotent cells"*

**作者**:Jørgensen S, et al.

**摘要**:利用H4K20me3抗体进行ChIP-seq分析,发现多能干细胞中H4K20me3的动态分布与基因沉默及染色质压缩密切相关,提示其参与早期发育调控。

3. **文献名称**:*"DNA damage triggers acute H4K20 methylation by SUV420H1 to ensure checkpoint activation"*

**作者**:Tuzon CT, et al.

**摘要**:通过Western blot和免疫荧光实验(使用H4K20me3抗体),证明DNA损伤后SUV420H1介导的H4K20三甲基化对细胞周期检查点激活至关重要。

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**备注**:HIST2H4A是组蛋白H4的编码基因之一,而H4K20me3是组蛋白H4第20位赖氨酸的三甲基化修饰。上述文献均聚焦于该修饰的功能研究,并明确使用了特异性抗体进行实验验证。若需针对HIST2H4A基因本身的抗体文献,可能需要更具体的基因表达或亚型研究。

背景信息

The HIST2H4A(20Me3) antibody specifically recognizes histone H4 trimethylated at lysine 20 (H4K20me3), a post-translational modification associated with transcriptional regulation and chromatin organization. Histones, including H4. undergo covalent modifications that influence DNA accessibility and gene expression. H4K20me3 is enriched in constitutive heterochromatin, telomeres, and specific silenced genomic regions, playing roles in maintaining genomic stability, DNA repair, and cell cycle progression. This mark is deposited by the SUV420H1 and SUV420H2 methyltransferases and is linked to the establishment of repressive chromatin states. Dysregulation of H4K20me3 has been implicated in cancer, developmental disorders, and aging-related processes. The HIST2H4A(20Me3) antibody is widely used in epigenetics research to study chromatin dynamics, particularly in models of disease or cellular differentiation. It is validated for applications such as chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting. Proper controls, including peptide competition or knockout cell lines, are essential to confirm specificity due to potential cross-reactivity with similar histone marks. This antibody serves as a critical tool for exploring the functional interplay between histone modifications, chromatin structure, and gene regulation.

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