Mouse Monoclonal HIST2H4A(20Me3) Antibody

The HIST2H4A(20Me3) antibody specifically detects histone H4 trimethylated at lysine 20 (H4K20me3), a post-translational modification critical for chromatin organization and genomic regulation. Histone H4. encoded by the HIST2H4A gene (part of the histone cluster on chromosome 1), is a core component of nucleosomes. H4K20me3 is enriched in heterochromatin regions and plays roles in DNA repair, replication timing, and transcriptional silencing. This mark is deposited by SUV420H1 and SUV420H2 methyltransferases and is associated with chromatin compaction, telomere stability, and mitotic regulation. Aberrant H4K20me3 levels are linked to cancer, developmental disorders, and genome instability. The HIST2H4A(20Me3) antibody is widely used in epigenetics research to study chromatin dynamics, cellular differentiation, and disease mechanisms via techniques like ChIP-seq, immunofluorescence, and Western blot. Its specificity for the trimethylated form helps distinguish active chromatin states from repressive ones. Validating this antibody is essential, as cross-reactivity with other methylated histone residues can occur. Understanding H4K20me3’s role provides insights into epigenetic therapies targeting chromatin-modifying enzymes in oncology and other fields.