| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | I340M |
| Uniprot No | P15151 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-343aa |
| 氨基酸序列 | WPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELTVQVKEGPPSEHSGMSRN |
| 预测分子量 | 64.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于I340M重组蛋白的假设性参考文献示例(请注意，以下文献为示例性内容，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*Functional Characterization of the I340M Mutation in Recombinant Voltage-Gated Sodium Channels*
**作者**：Zhang L, et al.
**摘要**：研究通过电生理学分析发现，Nav1.7钠通道的I340M突变导致通道激活阈值降低，可能与周围神经病变性疼痛相关。
2. **文献名称**：*Impact of I340M Substitution on Recombinant HIV-1 Protease Drug Resistance*
**作者**：Johnson R, et al.
**摘要**：该突变体表现出对多种蛋白酶抑制剂的耐药性增强，结构模拟显示突变改变了活性位点的疏水相互作用。
3. **文献名称**：*Crystallographic Analysis of Recombinant p53 I340M Mutant in Cancer Pathways*
**作者**：Kim S, et al.
**摘要**：I340M突变破坏p53 DNA结合域的结构稳定性，削弱其转录调控功能，促进肿瘤发生。
4. **文献名称**：*I340M Mutation Enhances Aggregation Propensity of Recombinant Tau Protein in Neurodegeneration*
**作者**：Martinez F, et al.
**摘要**：体外实验表明，该突变加速Tau蛋白纤维形成，可能与阿尔茨海默病的病理进程相关。
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**建议**：实际研究中，请通过**PubMed**、**Google Scholar**等平台，以关键词“I340M recombinant protein”或“I340M mutation [蛋白名称]”检索最新文献。若需特定蛋白背景，可进一步限定搜索范围(如“Nav1.5 I340M”)。
The I340M recombinant protein is a genetically engineered variant of a native protein, where the isoleucine residue at position 340 is replaced with methionine through site-directed mutagenesis. This substitution is typically designed to study the functional or structural role of this specific residue in the protein's activity, stability, or interactions. The parent protein's background varies depending on its origin; for instance, if derived from a receptor, enzyme, or signaling protein, the I340M mutation may be implicated in altering ligand binding, catalytic efficiency, or regulatory mechanisms. Such mutations are often explored in the context of disease-related pathways, such as cancer, neurological disorders, or metabolic dysfunctions, where amino acid changes correlate with pathological phenotypes.
Recombinant production of the I340M variant involves cloning the mutated gene sequence into expression vectors (e.g., bacterial, mammalian, or insect cell systems), followed by purification using affinity chromatography, size exclusion, or other techniques. Researchers employ this engineered protein to dissect structure-function relationships, validate hypotheses from computational models, or screen for targeted therapeutics. For example, in kinase studies, the I340M mutation might influence ATP-binding affinity or substrate recognition, providing insights into drug resistance mechanisms. In membrane proteins, this substitution could affect conformational dynamics or oligomerization.
The I340M recombinant protein serves as a critical tool for mechanistic studies, enabling comparisons with wild-type and other mutants to pinpoint molecular determinants of protein behavior. Its applications span biophysical assays, crystallography, and cell-based experiments, contributing to both basic research and drug development pipelines.
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