| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MEL; RAB8A |
| Entrez GeneID | 4218 |
| clone | 7G8B11 |
| WB Predicted band size | 23.7kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Rab8 (AA: 68-207) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于Rab8抗体的3篇参考文献示例(文献信息为模拟，非真实存在，供参考)：
1. **文献名称**：Rab8 GTPase regulates apical protein localization in epithelial cells
**作者**：Ang, A.L., et al.
**摘要**：研究使用Rab8特异性抗体进行免疫荧光和Western blot分析，揭示Rab8在调控上皮细胞顶端膜蛋白运输中的作用，证实其与极性建立相关。
2. **文献名称**：Rab8 modulates cytoskeletal reorganization during cell migration
**作者**：Sato, T., et al.
**摘要**：通过Rab8抗体阻断实验，发现Rab8与微管结合蛋白相互作用，促进细胞迁移过程中膜突起形成，为癌症转移机制提供新见解。
3. **文献名称**：Rab8 dysfunction links to Huntington’s disease pathology
**作者**：Li, X., et al.
**摘要**：利用Rab8抗体检测亨廷顿病模型中的蛋白异常聚集，证明Rab8介导的囊泡运输障碍可能导致神经元内毒性蛋白累积。
4. **文献名称**：Interaction of Rab8 with the exocyst complex in vesicle tethering
**作者**：Hattula, K., et al.
**摘要**：通过免疫共沉淀(使用Rab8抗体)揭示Rab8与exocyst复合物的结合，阐明其在后高尔基体囊栓锚定中的分子机制。
(注：以上文献为示例，实际引用需根据真实数据库检索结果。)
Rab8 antibodies are immunological tools specifically designed to detect Rab8. a small GTPase belonging to the Rab family of proteins involved in regulating intracellular membrane trafficking. Rab8 plays a critical role in vesicle transport, particularly in the recycling of cargo from the Golgi apparatus to the plasma membrane, and is essential for maintaining cell polarity, ciliogenesis, and signaling pathways. It cycles between an active GTP-bound state and an inactive GDP-bound state, interacting with effector proteins to coordinate membrane dynamics.
Antibodies targeting Rab8 are widely used in research to study its localization, expression levels, and functional interactions in various cell types. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate Rab8's role in diseases such as neurodegenerative disorders (e.g., Huntington’s disease), cancer metastasis, and ciliopathies. For example, Rab8 dysfunction has been linked to impaired ciliary formation, contributing to retinal degeneration or Bardet-Biedl syndrome.
These antibodies are typically developed in host species like rabbits or mice, and their specificity is validated using knockout cell lines or siRNA-mediated knockdown. Researchers rely on high-quality Rab8 antibodies to explore membrane trafficking mechanisms and their implications in health and disease, making them indispensable in cell biology and biomedical studies.
×
