| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BSG |
| Uniprot No | P35613 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 138-323aa |
| 氨基酸序列 | EPGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLA |
| 预测分子量 | 49.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于BSG(Basigin/CD147)重组蛋白的参考文献及其摘要内容概括：
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1. **文献名称**：*"Recombinant human basigin/CD147: production, purification, and structural characterization"*
**作者**：Zhang Y, et al.
**摘要**：该研究描述了通过哺乳动物表达系统(HEK293细胞)高效生产重组人源BSG蛋白的方法，并利用质谱和圆二色光谱验证其结构完整性，证明其与天然蛋白具有相似的糖基化修饰和构象特征。
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2. **文献名称**：*"Functional analysis of recombinant basigin in tumor cell invasion and angiogenesis"*
**作者**：Tang W, et al.
**摘要**：通过在大肠杆菌中表达BSG胞外域重组蛋白，研究发现其能够显著增强肿瘤细胞的迁移能力并促进血管内皮细胞管状结构形成，揭示了BSG通过调控基质金属蛋白酶(MMPs)分泌促进肿瘤侵袭的机制。
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3. **文献名称**：*"Expression and purification of bioactive basigin-2 in Pichia pastoris for antibody development"*
**作者**：Li H, et al.
**摘要**：利用毕赤酵母系统成功表达高纯度的BSG异构体(Basigin-2)重组蛋白，并证实其与单克隆抗体的特异性结合能力，为基于BSG的癌症诊断试剂开发提供了可靠抗原来源。
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(注：以上文献信息为基于领域研究的模拟概括，实际引用请以真实论文为准。)
**Background of BSG (Basigin) Recombinant Protein**
BSG (Basigin), also known as CD147 or EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It plays pivotal roles in diverse physiological and pathological processes, including tissue remodeling, immune response regulation, and cellular adhesion. Structurally, BSG contains two extracellular immunoglobulin (Ig)-like domains, a transmembrane region, and a short cytoplasmic tail. Its interaction partners include cyclophilins, integrins, and matrix metalloproteinases (MMPs), highlighting its function in modulating extracellular matrix degradation and cell signaling.
BSG is widely expressed in tissues such as the immune system, reproductive organs, and malignant tumors. In cancer, BSG is frequently overexpressed and correlates with poor prognosis. It promotes tumor progression by stimulating MMP production in stromal cells, facilitating angiogenesis, invasion, and metastasis. Additionally, BSG serves as a receptor for pathogens, including *Plasmodium falciparum* (malaria parasite) and SARS-CoV-2. where it assists viral entry by binding to the spike protein.
Recombinant BSG proteins are engineered for research and therapeutic applications. Produced via expression systems like *E. coli* or mammalian cells, these proteins retain functional domains for studying ligand-receptor interactions, antibody development, or inhibitor screening. In drug discovery, recombinant BSG aids in targeting cancer or infectious diseases by disrupting pathogenic interactions. Its role in COVID-19 has further spurred interest in developing BSG-based therapeutics. Despite challenges in structural complexity and glycosylation-dependent functions, recombinant BSG remains a critical tool for unraveling its multifaceted biology and translational potential.
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