| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ycbX |
| Uniprot No | P75863 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-369aa |
| 氨基酸序列 | MATLIRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGTFITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIAPDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQRCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQKHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAAADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQLLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR |
| 预测分子量 | 67.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ycbX重组蛋白的3-4条参考文献示例(注：内容为示例性概括，具体文献需根据实际数据库检索确认)：
1. **文献名称**：*Structural and functional characterization of the ycbX protein from Escherichia coli*
**作者**：Sainsbury, S., et al.
**摘要**：该研究解析了大肠杆菌ycbX重组蛋白的晶体结构，发现其具有金属结合位点，推测其参与锌离子稳态调节或细菌代谢相关通路。
2. **文献名称**：*Role of ycbX in bacterial biofilm formation and stress response*
**作者**：Wang, L., & Zhang, H.
**摘要**：通过基因敲除实验，发现ycbX缺失显著降低细菌生物膜形成能力，表明该蛋白可能在环境应激(如氧化压力)中发挥调控作用。
3. **文献名称**：*ycbX as a potential virulence factor in pathogenic bacteria*
**作者**：Martinez, R., et al.
**摘要**：研究显示，ycbX在沙门氏菌等致病菌中高表达，可能通过调控金属离子吸收增强细菌在宿主体内的存活和毒力。
4. **文献名称**：*Recombinant expression and enzymatic activity analysis of ycbX protein*
**作者**：Kumar, S., et al.
**摘要**：成功在大肠杆菌中表达并纯化ycbX重组蛋白，体外实验证实其具有金属依赖的酶活性，可能与肽酶或水解酶功能相关。
(注：以上文献信息为模拟生成，实际引用时需核实具体文献来源。)
The ycbX gene, found in various bacterial species including *Escherichia coli*, encodes a protein implicated in stress response and metabolic regulation. Though its exact biological role remains partially characterized, studies suggest ycbX may function as a metalloprotease or chaperone, potentially participating in zinc homeostasis or protein quality control under stress conditions. Its expression is regulated by the Zur (Zinc uptake regulator) protein, linking it to zinc limitation responses. In pathogenic bacteria like *Salmonella*, ycbX homologs contribute to virulence and biofilm formation, highlighting its potential role in bacterial adaptation to host environments.
Recombinant ycbX protein is typically produced using heterologous expression systems, such as *E. coli* expression strains, through cloning the gene into plasmid vectors with inducible promoters (e.g., T7 or arabinose promoters). Affinity tags like His-tags are commonly fused to facilitate purification via nickel-chelating chromatography. Structural studies using recombinant ycbX have revealed a conserved "MPP-like metalloprotease" domain architecture, though catalytic activity requires further validation.
Research on ycbX recombinant protein focuses on elucidating its enzymatic properties, metal-binding capabilities, and interactions with cellular substrates. It serves as a tool for investigating bacterial stress adaptation mechanisms and identifying potential antimicrobial targets. Recent work also explores its application in synthetic biology for metal ion sensing or engineered stress-response systems. Despite progress, functional ambiguity persists, driving ongoing investigations into its precise molecular mechanisms and ecological relevance across microbial species.
×
