| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DYT6 |
| Entrez GeneID | 55145 |
| clone | 8D10C8 |
| WB Predicted band size | 24.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human THAP1 (AA: 1-213) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于THAP1抗体的3篇模拟参考文献示例(实际文献需通过数据库核实)：
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1. **标题**: "Development and Characterization of a Specific Monoclonal Antibody for THAP1 Protein"
**作者**: Smith A, et al.
**摘要**: 本研究报道了一种新型抗THAP1单克隆抗体的开发，通过免疫印迹和免疫组化验证其特异性。抗体成功应用于检测人类和小鼠组织中THAP1的表达水平，并揭示其在神经元中的核定位，为研究THAP1相关神经疾病提供了工具。
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2. **标题**: "THAP1 Mutations in Dystonia: Loss-of-Function Mechanisms Analyzed via Antibody-Based Assays"
**作者**: Bressman SB, et al.
**摘要**: 通过使用THAP1特异性抗体，研究发现DYT6型肌张力障碍患者的突变导致THAP1蛋白稳定性下降及DNA结合能力受损。抗体在患者成纤维细胞中的免疫荧光分析证实了突变对亚细胞定位的影响。
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3. **标题**: "THAP1 Interactions with Chromatin Remodeling Complexes Revealed by ChIP-Seq"
**作者**: Campagne S, et al.
**摘要**: 利用THAP1抗体进行染色质免疫沉淀(ChIP)，揭示了THAP1与染色质重塑复合物(如NuRD)的相互作用，阐明其在基因转录调控中的分子机制，特别是在神经发育相关基因启动子区域的富集。
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如需真实文献，建议通过PubMed或Google Scholar以关键词“THAP1 antibody”、“THAP1 immunohistochemistry”或“THAP1 dystonia”检索近年研究。
THAP1 (THAP domain-containing protein 1) is a zinc finger transcription factor encoded by the *THAP1* gene, located on human chromosome 8. It regulates gene expression by binding to specific DNA sequences via its THAP domain, a conserved motif unique to this protein family. THAP1 plays a critical role in cell proliferation, apoptosis, and neuronal development. Mutations in *THAP1* are linked to DYT6 dystonia, a hereditary movement disorder characterized by involuntary muscle contractions.
THAP1 antibodies are immunological tools developed to detect and study the expression, localization, and functional interactions of THAP1 in research. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate THAP1's role in transcriptional regulation, its involvement in neurodevelopmental pathways, and the molecular mechanisms underlying dystonia. Studies using THAP1 antibodies have revealed its interaction with proteins like HCFC1 and its regulation of target genes such as *RRM1*.
Research with THAP1 antibodies also contributes to understanding how pathogenic mutations disrupt THAP1’s DNA-binding or protein-interaction capabilities, leading to cellular dysfunction. Their application extends to modeling disease mechanisms in vitro or in animal studies, aiding therapeutic discovery for dystonia and related neurological disorders. Validation of THAP1 antibodies remains essential to ensure specificity, given the protein’s structural similarities within the THAP family.
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