| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSTK1 |
| Uniprot No | Q9Y2Q3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-226aa |
| 氨基酸序列 | GPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL |
| 预测分子量 | 52.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GSTK1重组蛋白的模拟参考文献示例(内容仅供参考，具体文献需通过学术数据库查询)：
1. **文献名称**: *Recombinant expression and functional characterization of human GSTK1 in Escherichia coli*
**作者**: Zhang L, et al.
**摘要**: 本研究通过原核表达系统成功制备了具有活性的重组人GSTK1蛋白，验证其谷胱甘肽结合能力及抗氧化功能，为酶学机制研究提供工具。
2. **文献名称**: *Structural insights into GSTK1's role in mitochondrial redox regulation*
**作者**: Thompson R, et al.
**摘要**: 利用X射线晶体学解析了重组GSTK1的三维结构，揭示其与线粒体底物结合的关键位点，阐明了其在氧化应激中的分子作用机制。
3. **文献名称**: *GSTK1 overexpression attenuates hepatic lipid peroxidation in a murine model*
**作者**: Chen H, et al.
**摘要**: 通过腺病毒载体在肝脏中表达重组GSTK1.证明其可降低高脂饮食小鼠的脂质过氧化水平，提示其在代谢性疾病中的潜在治疗价值。
4. **文献名称**: *Comparative analysis of GSTK1 isoforms in detoxification pathways*
**作者**: Gupta S, et al.
**摘要**: 对比不同物种来源的重组GSTK1蛋白活性，发现其对外源性毒素的催化效率差异，为进化生物学和毒理学研究提供数据支持。
注：以上文献信息为示例性质，实际引用时请以权威数据库(如PubMed、Web of Science)检索结果为准。
GSTK1 (Glutathione S-Transferase Kappa 1) is a member of the glutathione S-transferase superfamily, enzymes critical for cellular detoxification and oxidative stress response. Unlike classical GSTs localized in the cytoplasm, GSTK1 is uniquely targeted to mitochondria and peroxisomes due to its N-terminal targeting sequence, reflecting its specialized role in organelle-specific metabolic pathways. It catalyzes the conjugation of glutathione to electrophilic substrates, facilitating the elimination of toxic compounds and protecting cellular components from oxidative damage.
The recombinant GSTK1 protein is engineered for research applications, typically expressed in *E. coli* or mammalian systems, and purified to retain enzymatic activity. Its structure includes a conserved GST N-terminal domain and a C-terminal domain unique to the kappa class, enabling substrate specificity. Studies highlight its involvement in lipid metabolism, redox homeostasis, and modulation of signaling pathways, linking it to metabolic disorders, neurodegenerative diseases, and cancer.
Research on recombinant GSTK1 has advanced understanding of mitochondrial antioxidant defenses and its interplay with peroxisomal pathways. It serves as a tool for investigating drug metabolism, toxicity mechanisms, and therapeutic targeting. Dysregulation of GSTK1 has been implicated in insulin resistance and mitochondrial dysfunction, underscoring its potential as a biomarker or therapeutic target. However, its precise physiological substrates and regulatory mechanisms remain under exploration, driving ongoing interest in structural and functional studies of this atypical GST isoform.
×
