| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EDNRB |
| Uniprot No | P24530 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-442aa |
| 氨基酸序列 | EERGFPPDRATPLLQTAEIMTPPTKTLWPKGSNASLARSLAPAEVPKGDRTAGSPPRTISPPPCQGPIEIKETFKYINTVVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILIASLALGDLLHIVIDIPINVYKLLAEDWPFGAEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIKGIGVPKWTAVEIVLIWVVSVVLAVPEAIGFDIITMDYKGSYLRICLLHPVQKTAFMQFYKTAKDWWLFSFYFCLPLAITAFFYTLMTCEMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTLYNQNDPNRCELLSFLLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKSCLCCWCQSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKYSSS |
| 预测分子量 | 49,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EDNRB重组蛋白的3篇代表性文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索获取)：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant EDNRB in a Cellular Model of Hirschsprung’s Disease"*
**作者**：Puffenberger, E.G. et al.
**摘要**：研究通过哺乳动物细胞系统表达了EDNRB重组蛋白，并验证其在先天性巨结肠症(HSCR)细胞模型中的功能。发现EDNRB重组蛋白可恢复突变细胞的信号传导缺陷，提示其潜在治疗价值。
2. **文献名称**：*"Structural Analysis of EDNRB Using Recombinant Protein Crystallography"*
**作者**：Sakurai, T. et al.
**摘要**：利用大肠杆菌表达系统纯化EDNRB重组蛋白，并通过X射线晶体学解析其三维结构，揭示了配体(内皮素)结合的关键结构域，为靶向药物设计提供依据。
3. **文献名称**：*"EDNRB Recombinant Protein Attenuates Melanoma Invasion via Modulation of ERK Signaling"*
**作者**：Bagnato, A. et al.
**摘要**：研究证明外源性EDNRB重组蛋白可通过抑制ERK磷酸化通路，降低黑色素瘤细胞的迁移和侵袭能力，提示其在癌症治疗中的应用潜力。
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**说明**：以上文献摘要概括了EDNRB重组蛋白在疾病模型、结构解析及癌症治疗中的研究。实际文献需通过PubMed、Web of Science等平台检索关键词(如“EDNRB recombinant protein”或“endothelin receptor B expression”)获取。
Endothelin receptor type B (EDNRB) is a G protein-coupled receptor (GPCR) that binds endothelin peptides, primarily endothelin-3 (ET-3), to regulate diverse physiological processes. It plays a critical role in embryonic development, particularly in the migration and differentiation of neural crest cells, which contribute to melanocyte formation, enteric nervous system (ENS) development, and cardiovascular structures. EDNRB signaling activates intracellular pathways like phospholipase C and MAPK, influencing cell proliferation, survival, and differentiation. Mutations in the EDNRB gene are associated with Hirschsprung disease (congenital aganglionic megacolon) and Waardenburg syndrome type 4. disorders characterized by pigmentation defects and ENS abnormalities.
Recombinant EDNRB protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional receptor domains or full-length proteins for research. This technology enables detailed studies of EDNRB’s structure, ligand-binding mechanisms, and interaction with signaling partners. Recombinant EDNRB is widely used in drug discovery to screen for agonists/antagonists targeting cardiovascular diseases, cancer (due to its role in tumor angiogenesis), and neurodevelopmental disorders. It also aids in elucidating pathogenic mechanisms of EDNRB-related genetic diseases and validating therapeutic strategies, such as receptor modulation or gene therapy. By providing a controlled, scalable source of the receptor, recombinant EDNRB accelerates both basic research and translational applications.
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