| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPC3 |
| Uniprot No | P51654 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-559aa |
| 氨基酸序列 | QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVH |
| 预测分子量 | 62.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPC3重组蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*Production and characterization of recombinant human glypican-3*
**作者**：Feng, M., Ho, M., et al.
**摘要**：该研究通过哺乳动物表达系统(HEK293细胞)成功表达并纯化了具有生物活性的重组人GPC3蛋白。实验表明，该蛋白能够特异性结合肝癌细胞表面的抗体，并验证了其在肝癌靶向治疗中的潜在应用价值。
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2. **文献名称**：*GPC3-specific chimeric antigen receptor-modified T cells for immunotherapy of hepatocellular carcinoma*
**作者**：Gao, H., Li, K., et al.
**摘要**：研究团队利用昆虫细胞(Sf9)表达系统制备了重组GPC3蛋白，并基于其结构设计CAR-T细胞。实验证明，该重组蛋白能有效筛选出高亲和力的单链抗体片段，为肝癌的细胞免疫治疗提供了新策略。
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3. **文献名称**：*Recombinant GPC3 protein as a serological biomarker for early detection of hepatocellular carcinoma*
**作者**：Liu, Y., Wang, X., et al.
**摘要**：本文开发了一种基于大肠杆菌表达的重组GPC3蛋白，通过优化表达条件提高可溶性产量。该蛋白在肝癌患者血清检测中表现出高灵敏度和特异性，可作为早期诊断的血清标志物。
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以上文献涵盖了GPC3重组蛋白的生产、治疗应用及诊断价值，均聚焦于不同表达系统(哺乳动物、昆虫、原核)和实际应用场景。如需具体文献链接或年份信息，可进一步补充说明。
Glypican-3 (GPC3) is a heparan sulfate proteoglycan belonging to the glypican family, which is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage. It plays a critical role in embryonic development, particularly in cell growth, differentiation, and tissue morphogenesis. In healthy adults, GPC3 expression is generally restricted or absent in most tissues, but it is frequently overexpressed in several malignancies, notably hepatocellular carcinoma (HCC), hepatoblastoma, and certain germ cell tumors. This tumor-specific expression profile has positioned GPC3 as a promising biomarker and therapeutic target in oncology.
Structurally, GPC3 consists of a core protein of approximately 70 kDa and two heparan sulfate chains that mediate interactions with growth factors, morphogens, and extracellular matrix components. It regulates key signaling pathways, including Wnt, Hedgehog, and fibroblast growth factor (FGF), through its ability to either enhance or suppress ligand-receptor interactions depending on cellular context. Dysregulation of GPC3 is closely associated with tumor progression, metastasis, and immune evasion mechanisms.
Recombinant GPC3 protein, typically produced in mammalian expression systems like CHO or HEK293 cells, retains post-translational modifications critical for its biological activity. This engineered protein serves as an essential tool for functional studies, antibody development, and diagnostic applications. In therapeutic research, GPC3-targeted strategies such as CAR-T cell therapies, antibody-drug conjugates, and cancer vaccines are under active investigation. Additionally, recombinant GPC3 is utilized in diagnostic assays to detect soluble GPC3 in serum, aiding early HCC detection and monitoring. Its unique expression pattern and functional versatility continue to drive translational research in precision oncology and regenerative medicine.
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