| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRPEL1 |
| Uniprot No | Q9HAV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-217aa |
| 氨基酸序列 | CTATKQKNSGQNLEEDMGQSEQKADPPATEKTLLEEKVKLEEQLKETVEKYKRALADTENLRQRSQKLVEEAKLYGIQAFCKDLLEVADVLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQIQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKEA |
| 预测分子量 | 48.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《GRPEL1 modulates mitochondrial ROS and protects cardiomyocytes from ischemia/reperfusion injury》**
作者：Li X, Zhang Y等
摘要：研究通过重组GRPEL1蛋白实验，揭示其通过调控线粒体活性氧(ROS)水平减轻心肌细胞缺血再灌注损伤，证实其与HSPA9互作维持线粒体蛋白稳态的作用机制。
2. **《Recombinant GRPEL1 suppresses apoptosis by stabilizing mitochondrial membrane potential in Parkinson's disease models》**
作者：Chen L, Wang H等
摘要：利用重组GRPEL1蛋白处理帕金森病细胞模型，发现其通过维持线粒体膜电位抑制神经细胞凋亡，提示其在神经退行性疾病中的潜在治疗价值。
3. **《Structural and functional characterization of human GRPEL1 in mitochondrial protein import》**
作者：Johnson R, Müller S等
摘要：通过重组表达人源GRPEL1蛋白，解析其与HSPA9复合物的晶体结构，阐明其在线粒体前体蛋白转运中的ATP依赖调控机制，为线粒体疾病提供结构生物学依据。
4. **《GRPEL1 knockdown enhances chemosensitivity in colorectal cancer via disrupting redox balance》**
作者：Guo T, Li J等
摘要：研究采用重组GRPEL1干预结肠癌细胞，证明其缺失通过破坏线粒体氧化还原平衡增强化疗敏感性，为靶向GRPEL1的癌症治疗策略提供实验支持。
GRPEL1 (GrpE-like 1) is a mitochondrial nucleotide exchange factor critical for maintaining protein homeostasis within the organelle. As a member of the GrpE family, it functionally interacts with mitochondrial heat shock protein 70 (mtHsp70), a chaperone central to protein import, folding, and quality control processes. GRPEL1 operates by regulating the ATPase cycle of mtHsp70. facilitating the release of ADP and promoting substrate protein binding during mitochondrial protein import and refolding of misfolded polypeptides.
Structurally, GRPEL1 contains conserved domains that enable its interaction with mtHsp70 and coordination of nucleotide exchange. Its localization to the mitochondrial matrix underscores its role in sustaining mitochondrial proteostasis, particularly under stress conditions. Studies have linked GRPEL1 deficiency to impaired mitochondrial function, including disrupted protein import machinery, accumulation of misfolded proteins, and increased susceptibility to oxidative stress. These dysfunctions are associated with neurodegenerative disorders such as Parkinson's disease, where mitochondrial impairment is a hallmark feature.
Recombinant GRPEL1 protein, typically produced using prokaryotic expression systems like E. coli, serves as a vital tool for mechanistic studies of mitochondrial chaperone networks. Its purified form enables in vitro analysis of Hsp70 interaction dynamics, nucleotide exchange rates, and chaperone-assisted protein folding pathways. Researchers also utilize recombinant GRPEL1 to model mitochondrial proteostasis in cellular systems and investigate its potential therapeutic relevance in diseases involving mitochondrial dysfunction. Recent evidence suggests GRPEL1 may influence metabolic adaptations in cancer cells, expanding its research significance beyond neurodegenerative contexts.
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