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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PROK1 |
| Uniprot No | P58294 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-105aa |
| 氨基酸序列 | AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF |
| 预测分子量 | 9.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PROK1重组蛋白的3篇参考文献概览：
1. **文献名称**: *Prokineticin 1 Signaling and Gene Regulation in Early Human Pregnancy*
**作者**: S. Battersby et al.
**摘要**: 研究揭示了重组PROK1蛋白在早期妊娠中通过激活MAPK通路调控子宫内膜基因表达，促进胚胎着床和血管生成的作用机制。
2. **文献名称**: *Recombinant Prokineticin 1 Induces Inflammatory Cytokine Expression in Endothelial Cells*
**作者**: K. Kisliouk et al.
**摘要**: 该文献报道了重组PROK1通过结合PKR1受体激活NF-κB通路，诱导内皮细胞产生IL-6、TNF-α等促炎因子，提示其在炎症相关血管疾病中的潜在作用。
3. **文献名称**: *PROK1 Promotes Angiogenesis Through Dual Activation of ERK and PI3K Pathways*
**作者**: M. Ferrara et al.
**摘要**: 研究通过体外实验证明重组PROK1蛋白同时激活ERK和PI3K/Akt通路，显著增强内皮细胞迁移和血管网络形成能力，为缺血性疾病治疗提供理论依据。
注：以上文献信息为模拟示例，实际引用需核对具体论文数据。建议通过PubMed、Web of Science检索关键词 "PROK1 recombinant" "Prokineticin-1 expression" 获取最新研究。
**Background of PROK1 Recombinant Protein**
Prokineticin 1 (PROK1), also known as endocrine gland-derived vascular endothelial growth factor (EG-VEGF), is a small secreted protein encoded by the *PROK1* gene. It belongs to the prokineticin family, which includes PROK2. and functions through binding to two G protein-coupled receptors, PKR1 and PKR2. Structurally, PROK1 consists of 86 amino acids, featuring a conserved N-terminal AVIT motif critical for receptor interaction and a C-terminal cysteine-rich domain stabilized by disulfide bonds, ensuring structural stability and bioactivity.
PROK1 plays multifaceted roles in angiogenesis, cell proliferation, and tissue development. It is highly expressed in steroidogenic organs (e.g., adrenal glands, ovaries, testes), reproductive tissues (e.g., placenta, endometrium), and the central nervous system. In reproductive biology, PROK1 regulates vascular remodeling during corpus luteum formation, placental development, and endometrial receptivity, essential for embryo implantation and pregnancy maintenance. In the nervous system, it influences neurogenesis, circadian rhythms, and pain perception.
Dysregulation of PROK1 is linked to pathologies such as cancer, inflammatory disorders, and reproductive disorders. Overexpression in tumors promotes angiogenesis and metastasis, highlighting its potential as a therapeutic target. Recombinant PROK1 protein, produced via *E. coli* or mammalian expression systems, retains native bioactivity and is widely used in research to elucidate its signaling mechanisms, assess receptor interactions, and explore therapeutic applications. Its study offers insights into novel treatments for conditions involving vascular dysfunction, infertility, and neurodegenerative diseases.
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