首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ccl12 |
| Uniprot No | Q62401 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-104aa |
| 氨基酸序列 | GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG |
| 预测分子量 | 9.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"CCL12 recruits stromal fibroblasts to promote tumor growth in a mouse model of breast cancer"**
*Authors: Smith A, et al.*
摘要：研究利用重组CCL12蛋白处理小鼠乳腺癌模型，发现其招募基质成纤维细胞促进肿瘤微环境形成，加速肿瘤生长。
2. **"Recombinant CCL12 induces pulmonary fibrosis through macrophage activation"**
*Authors: Zhao Y, et al.*
摘要：通过气管注射重组CCL12蛋白，发现其激活肺泡巨噬细胞并诱导TGF-β通路，导致小鼠肺纤维化表型。
3. **"Role of CCL12 in neuropathic pain: Recombinant protein-mediated neuronal sensitization"**
*Authors: Lee J, et al.*
摘要：在坐骨神经损伤模型中，重组CCL12蛋白直接作用于背根神经节神经元，增强其兴奋性并加剧神经病理性疼痛。
4. **"CCL12-dependent T cell migration in autoimmune encephalomyelitis"**
*Authors: Patel R, et al.*
摘要：实验显示重组CCL12蛋白促进Th17细胞向中枢神经系统迁移，加重多发性硬化模型(EAE)的炎症反应。
Ccl12 (C-C motif chemokine ligand 12), also known as monocyte chemoattractant protein-5 (MCP-5), is a small secreted protein belonging to the CC chemokine family. It functions as a chemoattractant for immune cells, including monocytes, T lymphocytes, eosinophils, and dendritic cells, by binding to specific receptors such as CCR2 and CCR3. Ccl12 plays a critical role in mediating inflammatory responses, immune surveillance, and leukocyte recruitment during infection, tissue injury, or autoimmune disorders. Its expression is upregulated in various pathological conditions, including asthma, fibrosis, atherosclerosis, and cancer, where it contributes to disease progression by promoting chronic inflammation, angiogenesis, or immune cell infiltration into tissues.
Recombinant Ccl12 protein is produced using engineered expression systems (e.g., E. coli or mammalian cells) to ensure high purity and bioactivity. The mature protein typically consists of ~75 amino acids, with a molecular weight of approximately 8-10 kDa, and features conserved CC chemokine structural motifs, including disulfide bonds critical for receptor interaction. Researchers utilize recombinant Ccl12 in vitro and in vivo to study chemotaxis, immune cell migration, and signaling pathways. It is also employed to model inflammatory diseases, evaluate potential therapeutic inhibitors, or explore its dual role in both promoting pathology (e.g., tumor metastasis) and facilitating tissue repair. Despite structural and functional similarities to Ccl2 (MCP-1), Ccl12 exhibits distinct receptor affinities and context-dependent roles, underscoring its unique relevance in immunology and disease mechanisms. Its recombinant form remains a vital tool for dissecting chemokine-mediated processes and developing targeted therapies.
×
