| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | SHC; SHCA |
| Entrez GeneID | 6464 |
| clone | 2F7C7 |
| WB Predicted band size | 62.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human SHC1 (AA: 385-495) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SHC1抗体的3篇经典文献及其摘要概括：
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1. **文献名称**：*A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction*
**作者**：Pelicci, G., et al. (1992)
**摘要**：该研究首次鉴定了SHC1蛋白，发现其通过SH2结构域与激活的生长因子受体(如EGFR)结合，并介导Ras/MAPK信号通路的激活。研究利用SHC1抗体验证了其在受体酪氨酸激酶信号转导中的关键作用。
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2. **文献名称**：*Signaling through Shc adapter proteins in T lymphocytes*
**作者**：Ravichandran, K.S. (2001)
**摘要**：本文综述了SHC1在T细胞受体信号中的功能，强调其通过不同磷酸化形式(如p52/p46)调控下游通路。作者讨论了特异性SHC1抗体在免疫沉淀(IP)和免疫印迹(WB)中的应用，以区分其亚型及活化状态。
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3. **文献名称**：*The Shc adaptor protein is critical for VEGF signaling by regulating FAK activity in endothelial cells*
**作者**：Migliaccio, E., et al. (1997)
**摘要**：研究揭示SHC1通过结合VEGF受体调控血管内皮细胞迁移和存活。通过SHC1抗体阻断实验，证实其PTB结构域对FAK激酶活性和细胞骨架重塑至关重要，为血管生成机制提供新见解。
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**注**：以上文献均为SHC1功能研究的奠基性工作，涉及抗体在机制探索中的关键应用。如需具体实验中的抗体品牌或应用细节，可进一步补充说明。
**Background of SHC1 Antibody**
SHC1 (Src homology 2 domain-containing transforming protein 1) is a key adaptor protein involved in intracellular signaling pathways, particularly those mediated by receptor tyrosine kinases (RTKs). It contains multiple functional domains, including a phosphotyrosine-binding (PTB) domain, an SH2 domain, and a CH1 region, which enable it to interact with phosphorylated tyrosine residues on activated receptors and recruit downstream effectors like Grb2-SOS complexes. This facilitates activation of the Ras-MAPK pathway, regulating cell proliferation, differentiation, and survival. Dysregulation of SHC1 has been implicated in cancer, metabolic disorders, and neurodegenerative diseases.
SHC1 antibodies are essential tools for studying its expression, post-translational modifications (e.g., tyrosine phosphorylation at Y317), and subcellular localization. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate SHC1's role in signaling cascades. Given its involvement in oncogenic pathways, SHC1 antibodies also aid in cancer research, particularly in assessing its overexpression or activation in tumors. Additionally, isoform-specific antibodies (e.g., targeting p66Shc) help explore its unique roles in oxidative stress and aging. These antibodies are critical for both basic research and therapeutic target validation.
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