| WB | 1/500 - 1/2000 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/100 - 1/500 | Human,Mouse,Rat,Rabbit |
| ICC | 技术咨询 | Human,Mouse,Rat,Rabbit |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Aliases | PKR; PRKR; EIF2AK1 |
| Entrez GeneID | 5610 |
| clone | 6H3A10 |
| WB Predicted band size | 62kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human EIF2AK2 (AA: 329-551) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于EIF2AK2(PKR)抗体的3篇参考文献示例，涵盖其应用及研究背景：
1. **"Monoclonal antibodies targeting the dsRNA-binding domain of PKR reveal its activation dynamics in viral infection"**
*Authors: Williams, B.R., et al.*
**摘要**：该研究开发了针对PKR双链RNA结合结构域的单克隆抗体，验证其在Western blot和免疫荧光中的应用，揭示了PKR在病毒感染早期的磷酸化激活过程。
2. **"Antibody-based detection of phosphorylated PKR in neurodegenerative disease models"**
*Authors: García, M.A., et al.*
**摘要**：通过特异性抗磷酸化PKR抗体，研究者在阿尔茨海默病模型中观察到PKR的异常激活，表明其与神经元应激反应和tau蛋白病理相关。
3. **"Validation of a commercial EIF2AK2 antibody for immunohistochemical analysis in breast cancer"**
*Authors: Kim, S.H., et al.*
**摘要**：验证某商业抗体的特异性，发现EIF2AK2在乳腺癌组织中的高表达与化疗耐药性和患者预后不良显著相关。
4. **"PKR inhibition by a small molecule antagonist: antibody validation in kinase activity assays"**
*Authors: Smith, J., et al.*
**摘要**：利用EIF2AK2抗体验证激酶抑制剂的效果，证明其在体外和体内模型中有效阻断PKR活性，为抗病毒治疗提供新策略。
---
*注：上述文献为示例性质，实际引用需根据具体研究查询真实数据库(如PubMed)。如需真实文献，建议以“EIF2AK2 antibody”、“PKR antibody application”为关键词检索相关论文。*
The EIF2AK2 antibody targets the eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), also known as protein kinase R (PKR). This serine/threonine kinase is a key component of the innate immune response, primarily activated by double-stranded RNA (dsRNA) during viral infections. Upon activation, EIF2AK2 phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), leading to global translational arrest to limit viral replication. Beyond its antiviral role, EIF2AK2 regulates stress signaling, apoptosis, inflammation, and cellular homeostasis, with implications in cancer, neurodegenerative diseases, and metabolic disorders.
EIF2AK2 antibodies are essential tools for studying its expression, activation, and interaction networks. They enable detection of EIF2AK2 in Western blotting, immunohistochemistry, and immunofluorescence, helping to elucidate its tissue-specific distribution and subcellular localization. Additionally, these antibodies are used in functional assays to investigate phosphorylation-dependent regulatory mechanisms or dsRNA-mediated signaling pathways. Research applications span virology, oncology, and immunology, particularly in exploring how dysregulated EIF2AK2 activity contributes to chronic inflammation, tumor progression, or viral immune evasion. Commercial EIF2AK2 antibodies are typically validated for specificity across human, mouse, and rat models, with monoclonal and polyclonal variants available to suit experimental needs.
×
