| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDHK2; PDKII |
| Entrez GeneID | 5164 |
| clone | 3F2C4 |
| WB Predicted band size | 46.2kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDK2 (AA: 178-404) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PDK2抗体的参考文献示例(内容为模拟生成，建议通过学术数据库查询具体文献)：
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1. **"Development and Validation of a Specific PDK2 Monoclonal Antibody for Metabolic Studies"**
*作者：Smith J, et al.*
摘要：本研究开发了一种高特异性的抗PDK2单克隆抗体，并通过Western blot和免疫组化验证其有效性。抗体成功应用于检测小鼠肝脏和心脏组织中PDK2的表达水平，证实其在代谢调控研究中的实用性。
2. **"PDK2 Expression in Cancer: Role of a Novel Polyclonal Antibody in Tumor Profiling"**
*作者：Lee H, et al.*
摘要：文章报道了一种新型PDK2多克隆抗体的开发，并用于分析多种癌症细胞系中PDK2的差异表达。研究揭示了PDK2在肿瘤糖代谢重编程中的作用，抗体特异性通过siRNA敲低实验验证。
3. **"Antibody-Based Detection of PDK2 in Diabetic Models: Implications for Insulin Resistance"**
*作者：Garcia R, et al.*
摘要：通过商业化PDK2抗体(如CST #ABC123)，本研究在糖尿病小鼠模型中检测PDK2蛋白水平变化，发现其与胰岛素信号通路异常相关，抗体特异性通过肽段竞争实验确认。
4. **"A Comparative Study of PDK2 Antibody Performance in Neurodegenerative Disease Models"**
*作者：Wang T, et al.*
摘要：比较了多种市售PDK2抗体在阿尔茨海默病脑组织样本中的检测效果，推荐抗体XYZ品牌在神经组织中的高灵敏度和低交叉反应性，为PDK2与神经代谢研究提供工具支持。
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建议通过 **PubMed、Google Scholar** 或 **Web of Science** 搜索关键词“PDK2 antibody + application/validation”获取真实文献。如需具体文献DOI或期刊信息，请提供更详细的研究背景。
The pyruvate dehydrogenase kinase 2 (PDK2) antibody is a crucial tool for studying the regulation of cellular energy metabolism. PDK2 is a mitochondrial serine/threonine kinase that phosphorylates and inactivates the pyruvate dehydrogenase complex (PDC), a key enzyme converting pyruvate to acetyl-CoA for the tricarboxylic acid (TCA) cycle. By suppressing PDC activity, PDK2 promotes a metabolic shift from glucose oxidation to glycolysis, a mechanism often exploited by cancer cells (Warburg effect) and implicated in metabolic disorders like diabetes.
PDK2 antibodies are widely used in research to detect PDK2 expression, localization, and activity in tissues or cells. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into PDK2's role in diseases. For instance, elevated PDK2 levels correlate with tumor progression, insulin resistance, and hypoxia adaptation. These antibodies also support therapeutic exploration, as PDK2 inhibition is being studied to reactivate mitochondrial glucose oxidation in cancer or metabolic syndromes.
The specificity and sensitivity of PDK2 antibodies are critical, given the structural similarities among PDK isoforms (PDK1. 3. 4). Validated antibodies help dissect isoform-specific functions, advancing our understanding of metabolic reprogramming in health and disease.
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