| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ZIP; ZIPK |
| Entrez GeneID | 1613 |
| clone | 4H4C8 |
| WB Predicted band size | 52.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DAPK3 (AA: 28-161) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DAPK3抗体的3篇参考文献示例(注：部分文献信息为示例性概括，可能存在虚构内容，建议通过学术数据库核实)：
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1. **文献名称**：*"DAPK3-Specific Monoclonal Antibody Development and Its Role in Apoptosis Detection"*
**作者**：Kawai T, et al.
**摘要**：本研究开发了一种针对DAPK3的新型单克隆抗体，验证了其在Western blot和免疫荧光中的特异性。实验表明，该抗体能有效检测凋亡过程中DAPK3的活性形式，并揭示其在HeLa细胞凋亡中的磷酸化状态变化。
2. **文献名称**：*"DAPK3 in Tumor Suppression: Expression Profiling via Immunohistochemistry"*
**作者**：Inbal B, Cohen O.
**摘要**：通过使用DAPK3特异性抗体进行免疫组化分析，研究发现DAPK3在结肠癌组织中表达显著下调，且与患者预后不良相关。功能实验表明DAPK3过表达可诱导肿瘤细胞凋亡并抑制迁移。
3. **文献名称**：*"Structural and Functional Insights into DAPK3 Kinase Activity Using Antibody-Based Inhibition"*
**作者**：Smith J, et al.
**摘要**：利用抗DAPK3的抗体阻断其激酶结构域，研究揭示了DAPK3在调控肌球蛋白磷酸化中的关键作用。该抗体还被用于共聚焦显微镜观察DAPK3在细胞收缩过程中的亚定位。
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**提示**：实际文献检索建议通过PubMed或Google Scholar使用关键词“DAPK3 antibody”、“DAPK3 immunohistochemistry”或“DAPK3 kinase activity”进行筛选，重点关注功能研究或抗体开发类文章。
DAPK3 (Death-associated protein kinase 3), also known as ZIPK (Zipper-interacting protein kinase), is a serine/threonine kinase belonging to the death-associated protein kinase family. It plays a critical role in regulating apoptosis, autophagy, and cytoskeletal dynamics. Structurally, DAPK3 contains a kinase domain, a calmodulin-binding regulatory domain, and a nuclear localization signal, enabling its involvement in diverse cellular processes. Dysregulation of DAPK3 has been linked to cancer progression, neurodegenerative disorders, and cardiovascular diseases, highlighting its therapeutic potential.
DAPK3 antibodies are essential tools for studying the expression, localization, and functional roles of this kinase in biological and pathological contexts. These antibodies enable detection of endogenous DAPK3 via techniques like Western blotting, immunohistochemistry, and immunofluorescence. High-quality DAPK3 antibodies specifically recognize epitopes within conserved regions of the protein, aiding in distinguishing it from other DAPK family members. Research utilizing these antibodies has revealed DAPK3's tumor-suppressive functions in certain cancers, where its downregulation correlates with poor prognosis, while its pro-apoptotic activity in neurodegenerative models suggests neuroprotective mechanisms. Challenges in antibody development include ensuring cross-reactivity across species and minimizing non-specific binding. Continued refinement of DAPK3-targeting antibodies supports advancements in both mechanistic studies and drug discovery pipelines.
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