| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CHOP; CEBPZ; CHOP10; CHOP-10; GADD153 |
| Entrez GeneID | 1649 |
| clone | 4E8E9 |
| WB Predicted band size | 19.2kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DDIT3 (AA: 87-192) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DDIT3抗体的3篇参考文献示例(文献信息为模拟虚构，仅作格式参考)：
1. **文献名称**: "DDIT3/CHOP Antibody Specificity in Endoplasmic Reticulum Stress Models"
**作者**: Smith A, et al.
**摘要**: 本研究验证了DDIT3抗体在检测内质网应激诱导的CHOP蛋白表达中的特异性，通过Western blot和免疫组化分析，证实其在多种细胞模型中的可靠性。
2. **文献名称**: "Role of CHOP in Apoptosis: Validation of a Commercial DDIT3 Antibody"
**作者**: Lee J, et al.
**摘要**: 文章评估了市售DDIT3抗体(货号AB123)在神经退行性疾病模型中的应用，证明其在小鼠脑组织切片中检测CHOP蛋白的敏感性，并关联了凋亡通路激活。
3. **文献名称**: "DDIT3 as a Biomarker in Breast Cancer: Antibody Comparison Study"
**作者**: Garcia R, et al.
**摘要**: 比较了不同来源的DDIT3抗体在乳腺癌组织中的表现，发现某品牌抗体(如Santa Cruz sc-7351)在免疫荧光和流式细胞术中具有高特异性，可用于预后标志物研究。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed。)
**Background of DDIT3 Antibody**
The DDIT3 (DNA Damage Inducible Transcript 3) antibody is a crucial tool for detecting the DDIT3 protein, also known as CHOP (CCAAT/enhancer-binding protein homologous protein) or GADD153 (growth arrest and DNA damage-inducible protein 153). DDIT3 is a stress-inducible nuclear protein encoded by the *DDIT3* gene, belonging to the C/EBP family of transcription factors. It is upregulated in response to cellular stressors such as endoplasmic reticulum (ER) stress, DNA damage, oxidative stress, or nutrient deprivation, primarily through the unfolded protein response (UPR) and the integrated stress response (ISR) pathways.
DDIT3 plays a dual role in cellular fate: it promotes apoptosis under prolonged stress by regulating pro-apoptotic genes (e.g., *BIM*, *TRIB3*) while suppressing anti-apoptotic pathways. Its expression is closely associated with pathological conditions, including cancer, neurodegenerative diseases, diabetes, and metabolic disorders. The DDIT3 antibody is widely used in research to investigate ER stress-mediated apoptosis, tumor progression, and mechanisms of diseases linked to proteostatic imbalance.
Available as monoclonal or polyclonal antibodies, DDIT3-specific reagents are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Their specificity ensures accurate detection of DDIT3's ~29 kDa protein, aiding in studies of stress signaling and therapeutic targeting in disease models.
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