| WB | 1/500 - 1/2000 | Human,Mouse,Monkey,Rat |
| IF | 咨询技术 | Human,Mouse,Monkey,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Monkey,Rat |
| ICC | 技术咨询 | Human,Mouse,Monkey,Rat |
| FCM | 咨询技术 | Human,Mouse,Monkey,Rat |
| Elisa | 1/10000 | Human,Mouse,Monkey,Rat |
| Aliases | RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA |
| Entrez GeneID | 1432 |
| clone | 10B11C8 |
| WB Predicted band size | 41.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey,Rat |
| Immunogen | Purified recombinant fragment of human MAPK14 (AA: 299-360) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MAPK14抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
1. **文献名称**：*"Selective inhibition of p38 MAPK by a novel antibody-based strategy attenuates cardiac inflammation and fibrosis in hypertension"*
**作者**：Li et al.
**摘要**：本研究开发了一种特异性靶向MAPK14(p38α)的单克隆抗体，验证其在高血压模型中的抗炎和抗纤维化作用。通过免疫印迹和免疫组化证实抗体对p38α的高选择性，显著降低心脏炎症因子表达，提示其治疗潜力。
2. **文献名称**：*"MAPK14 phosphorylation status determines cellular fate in response to oxidative stress"*
**作者**：Chen & Davis
**摘要**：利用MAPK14磷酸化特异性抗体(如抗p-p38α)，发现氧化应激下MAPK14的激活程度调控细胞凋亡与存活。研究通过流式细胞术和免疫荧光验证抗体特异性，揭示其在细胞应激反应中的动态变化。
3. **文献名称**：*"A functional screen identifies kinases driving cancer stem cell self-renewal via MAPK14"*
**作者**：Guo et al.
**摘要**：通过CRISPR筛选结合MAPK14抗体(Western blot和ChIP验证)，发现MAPK14通过调控SOX2转录促进肿瘤干细胞增殖。研究强调该抗体在靶向治疗中的诊断价值。
以上文献涵盖抗体开发、信号机制及疾病应用，适用于实验设计参考。如需更多细节，建议通过PubMed或期刊数据库查询全文。
MAPK14 (mitogen-activated protein kinase 14), also known as p38α, is a serine/threonine kinase within the p38 MAPK family, playing a central role in cellular stress responses, inflammation, apoptosis, and differentiation. It is activated by environmental stressors (e.g., UV radiation, osmotic shock) and cytokines, triggering phosphorylation cascades that regulate downstream targets, including transcription factors (e.g., ATF2. TP53) and kinases. Dysregulation of MAPK14 signaling is implicated in cancer, neurodegenerative diseases, and autoimmune disorders, making it a therapeutic target.
MAPK14 antibodies are essential tools for studying its expression, activation, and localization. These antibodies typically detect endogenous MAPK14 or its phosphorylated forms (e.g., Thr180/Tyr182) to assess activation status. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). High-quality antibodies exhibit specificity validated via knockout controls or competitive peptides. Some antibodies target distinct epitopes, such as the N-terminal region or kinase domain, enabling diverse experimental applications.
Researchers utilize MAPK14 antibodies to explore its role in disease mechanisms, drug response, and pathway interactions. For instance, they help evaluate p38α inhibition in inflammatory models or correlate its expression with clinical outcomes in cancer. Cross-reactivity with other p38 isoforms (e.g., p38β, γ, δ) should be ruled out to ensure data accuracy. Overall, MAPK14 antibodies are pivotal in both basic research and translational studies targeting MAPK-driven pathologies.
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