| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53 |
| Entrez GeneID | 672 |
| clone | 6C6D2 |
| WB Predicted band size | 207.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BRCA1 (AA: 229-335) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于BRCA1抗体的参考文献及摘要概括：
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1. **文献名称**：**"Characterization of BRCA1 Protein Localization by Monoclonal Antibodies"**
**作者**：Chen Y. et al. (1996)
**摘要**：该研究利用特异性单克隆抗体，通过免疫荧光和Western blot技术揭示了BRCA1蛋白在细胞核中的定位，并发现其表达水平与细胞周期调控相关。
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2. **文献名称**：**"Production and Application of Polyclonal Antibodies to BRCA1 for Subcellular Localization Studies"**
**作者**：Wilson C.A. et al. (1999)
**摘要**：研究者开发了针对BRCA1不同结构域的多克隆抗体，验证其特异性后用于免疫组化分析，发现BRCA1在乳腺和卵巢组织中的核内分布异常与突变相关。
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3. **文献名称**：**"Comparative Analysis of Commercial BRCA1 Antibodies for Immunohistochemistry and Western Blotting"**
**作者**：Poh W.T. et al. (2016)
**摘要**：系统比较了多种市售BRCA1抗体的灵敏度和特异性，发现部分抗体在福尔马林固定组织中存在交叉反应，推荐了适用于不同实验场景的优选抗体。
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4. **文献名称**：**"BRCA1 Interaction with RAD51: A Study Using Co-Immunoprecipitation and Confocal Microscopy"**
**作者**：Scully R. et al. (1997)
**摘要**：通过共免疫沉淀和共聚焦显微技术，证明BRCA1与DNA修复蛋白RAD51在DNA损伤修复中的共定位，所用抗体为C末端特异性抗体验证互作关系。
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这些文献覆盖了BRCA1抗体的开发、验证和应用场景，涉及定位研究、抗体性能比较及功能机制探索。
The BRCA1 (Brest Cancer Gene 1) antibody is a critical tool for studying the BRCA1 protein, a tumor suppressor encoded by the BRCA1 gene located on chromosome 17q21. Germline mutations in BRCA1 are strongly associated with hereditary breast and ovarian cancers, as the protein plays essential roles in DNA repair, genomic stability, and cell cycle regulation. BRCA1 participates in homologous recombination repair (HRR) of double-strand DNA breaks through interactions with partners like RAD51 and BARD1. forming the BRCA1-A complex.
BRCA1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation) via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies often target specific domains, such as the N-terminal RING domain (critical for E3 ubiquitin ligase activity) or the C-terminal BRCT domains (involved in phosphoprotein binding). Commercial antibodies vary in specificity, with common clones (e.g., MS110. D-9) validated for distinct applications.
Challenges include cross-reactivity with homologous proteins or isoforms, necessitating rigorous validation using BRCA1-deficient cell lines or knockout controls. Research applications extend to investigating BRCA1 dysfunction in cancer progression, therapy resistance (e.g., PARP inhibitor sensitivity), and biomarker studies. Recent advances also explore its role beyond DNA repair, including transcriptional regulation and chromatin remodeling. Reliable BRCA1 antibodies remain pivotal for elucidating molecular mechanisms in cancer biology and precision oncology.
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