| WB | 1/500 - 1/2000 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/100 - 1/500 | Human,Mouse,Rat,Rabbit |
| ICC | 1/50 - 1/500 | Human,Mouse,Rat,Rabbit |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Aliases | SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38 |
| Entrez GeneID | 7291 |
| clone | 10E4E6 |
| WB Predicted band size | 21kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与TWIST1抗体相关的文献摘要示例(文献为虚构，仅供格式参考)：
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1. **文献名称**: "TWIST1 Antibody Validation in Epithelial-Mesenchymal Transition Models"
**作者**: Lee et al.
**摘要**: 本研究通过免疫印迹(Western Blot)和免疫组化(IHC)验证了TWIST1抗体在乳腺癌细胞系中的特异性，证实其在EMT过程中高表达，并发现抗体可有效检测转移性肿瘤组织中的TWIST1核定位。
2. **文献名称**: "Role of TWIST1 in Cancer Stem Cell Maintenance: Antibody-Based Functional Analysis"
**作者**: Gupta et al.
**摘要**: 利用TWIST1特异性抗体进行染色质免疫沉淀(ChIP)，揭示了TWIST1直接调控癌症干细胞相关基因的机制，抗体特异性通过siRNA敲低实验得到验证。
3. **文献名称**: "Comparative Study of Commercial TWIST1 Antibodies in Pancreatic Cancer"
**作者**: Martinez et al.
**摘要**: 对比了5种市售TWIST1抗体的灵敏度和特异性，推荐抗体Clone 7B2适用于免疫荧光(IF)检测，其在胰腺癌组织切片中表现出低背景和高信噪比。
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如需真实文献，建议在PubMed或Google Scholar中搜索关键词“TWIST1 antibody validation”或“TWIST1 immunohistochemistry”获取最新研究。
The TWIST1 antibody is a crucial tool for studying the TWIST1 protein, a basic helix-loop-helix (bHLH) transcription factor pivotal in embryonic development and cancer progression. TWIST1 regulates cell differentiation, migration, and tissue patterning during embryogenesis, particularly in mesoderm formation and neural crest cell development. In cancer research, TWIST1 is a key driver of epithelial-mesenchymal transition (EMT), a process enabling tumor cells to acquire invasive properties and metastasize. Its overexpression is linked to aggressive tumor behavior, therapy resistance, and poor prognosis in carcinomas such as breast, prostate, and ovarian cancers.
TWIST1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression levels and localization in cells or tissues. These antibodies aid in elucidating TWIST1's role in developmental biology, tumor microenvironment interactions, and metastatic pathways. Researchers also employ them to explore TWIST1's interplay with other signaling molecules, such as Wnt/β-catenin and TGF-β.
Available as monoclonal or polyclonal variants, TWIST1 antibodies vary in specificity and application. Monoclonal antibodies offer high reproducibility for consistent experimental results, while polyclonal versions may enhance sensitivity for detecting low-abundance targets. Validation via knockout/knockdown controls or recombinant protein assays is critical to ensure antibody reliability. Given TWIST1's dual role in development and disease, its antibodies remain indispensable for both basic research and translational studies aiming to target TWIST1 in anticancer therapies.
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