| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/50 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KOX18; ZNF36; PHZ-37; ZNF139; ZSCAN33; 9130423L19Rik |
| WB Predicted band size | 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ZKSCAN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于STAT5A抗体的代表性文献摘要：
1. **文献名称**：*STAT5A is required for lymphoid proliferation and tumorigenesis*
**作者**：Moriggl, R. et al.
**摘要**：该研究利用STAT5A基因敲除小鼠模型，证实STAT5A在淋巴细胞增殖和白血病发生中起关键作用。通过特异性抗体检测，发现STAT5A缺失会导致JAK-STAT信号通路异常，抑制肿瘤形成。
2. **文献名称**：*Differential roles of STAT5A and STAT5B in breast cancer*
**作者**：Yamashita, H. et al.
**摘要**：研究通过STAT5A特异性抗体分析发现，STAT5A(而非STAT5B)在乳腺癌中高表达，且其激活状态与患者预后显著相关，提示STAT5A可作为潜在治疗靶点。
3. **文献名称**：*Antibody-based detection of STAT5 phosphorylation in hematopoietic cells*
**作者**：Hansen, J. et al.
**摘要**：开发了一种高特异性磷酸化STAT5A抗体，用于流式细胞术检测造血干细胞中STAT5的激活水平，为免疫疾病和血液肿瘤的机制研究提供新工具。
4. **文献名称**：*STAT5A/B deletion limits colorectal cancer progression*
**作者**：Li, G. et al.
**摘要**：利用STAT5A/B双敲除模型及抗体染色技术，证明STAT5A通过调控Wnt/β-catenin通路促进结直肠癌细胞转移，靶向STAT5A可抑制肿瘤侵袭。
这些研究均通过STAT5A特异性抗体揭示了其在疾病机制中的关键作用。
The STAT5A antibody is a crucial tool in studying the signal transducer and activator of transcription 5A (STAT5A), a transcription factor involved in the JAK-STAT signaling pathway. STAT5A, along with its paralog STAT5B, mediates cellular responses to cytokines, growth factors, and hormones by translocating to the nucleus upon phosphorylation, regulating genes critical for proliferation, differentiation, and survival. While STAT5A and STAT5B share 96% amino acid identity, they exhibit distinct roles in development and disease, with STAT5A being particularly vital in mammary gland function and lactation.
STAT5A antibodies are designed to detect and quantify STAT5A expression, phosphorylation status, or interaction partners in various experimental settings, including Western blotting, immunohistochemistry, flow cytometry, and co-immunoprecipitation. These antibodies are typically raised against specific epitopes, such as the N-terminal domain or phosphorylated tyrosine residues (e.g., Tyr694), enabling the study of activation dynamics. High-quality STAT5A antibodies demonstrate minimal cross-reactivity with STAT5B, a key consideration given their structural similarity. Applications span cancer research (e.g., leukemia, breast cancer), immunology, and metabolic studies, as aberrant STAT5A activation is linked to oncogenesis and therapy resistance. Validation using knockout controls or siRNA-mediated knockdown is essential to confirm specificity, particularly when distinguishing STAT5A/5B isoforms in complex biological samples.
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