| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PLK; STPK13 |
| Entrez GeneID | 5347 |
| clone | 1D1 |
| WB Predicted band size | 68kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于PLK1抗体的参考文献(示例为虚构内容，仅作格式参考)：
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1. **文献名称**: *PLK1 Expression in Cancer: A Biomarker and Therapeutic Target*
**作者**: Smith J, et al.
**摘要**: 该研究通过PLK1抗体检测多种癌细胞系及肿瘤组织中的PLK1蛋白表达，发现PLK1在乳腺癌和结直肠癌中显著高表达，并验证其作为预后标志物的潜力。
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2. **文献名称**: *Development of a Novel Anti-PLK1 Antibody for Kinase Activity Analysis*
**作者**: Lee H, et al.
**摘要**: 研究团队开发了一种高特异性PLK1单克隆抗体，用于免疫沉淀和激酶活性检测，证明其在抑制PLK1磷酸化功能中的作用，为药物筛选提供工具。
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3. **文献名称**: *PLK1 Inhibition and Antibody-Based Targeting in Glioblastoma*
**作者**: Zhang R, et al.
**摘要**: 利用PLK1抗体研究胶质母细胞瘤中PLK1的定位及功能，结合小分子抑制剂实验，证明靶向PLK1可显著抑制肿瘤细胞增殖并诱导凋亡。
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4. **文献名称**: *Dynamic Localization of PLK1 During Mitosis Revealed by Immunofluorescence*
**作者**: Tanaka K, et al.
**摘要**: 通过免疫荧光技术结合PLK1抗体，系统性分析了PLK1在细胞有丝分裂不同阶段的亚细胞定位变化，揭示了其调控纺锤体组装的关键机制。
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(注：以上文献为示例，实际引用需检索PubMed或Google Scholar等平台获取真实文献。)
Polo-like kinase 1 (PLK1) is a serine/threonine kinase critical for regulating cell cycle progression, particularly during the G2/M phase and mitosis. It plays essential roles in centrosome maturation, spindle assembly, chromosome segregation, and cytokinesis. PLK1 is characterized by a conserved N-terminal kinase domain and a C-terminal Polo-box domain (PBD), which mediates substrate interactions and subcellular localization. Dysregulation of PLK1 is associated with genomic instability and tumorigenesis, making it a prominent therapeutic target in cancer research.
PLK1 antibodies are immunological tools designed to detect and quantify PLK1 expression, phosphorylation status, or localization in cells and tissues. These antibodies are widely used in techniques like Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry. Phospho-specific PLK1 antibodies, for instance, can identify activated PLK1 (e.g., phosphorylated at Thr210), providing insights into its functional state during mitosis.
In cancer biology, PLK1 is overexpressed in various malignancies, correlating with poor prognosis. PLK1 inhibitors (e.g., volasertib) are under clinical investigation, and corresponding antibodies help evaluate drug efficacy or resistance mechanisms. Researchers also use PLK1 antibodies to study its interactions with substrates (e.g., CDC25. Cyclin B1) and roles in checkpoint regulation. Validation of these antibodies remains crucial, as off-target binding or batch variability can affect experimental reproducibility. Overall, PLK1 antibodies are indispensable for dissecting mitotic mechanisms and advancing cancer therapeutics.
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