| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OF; PU.1; SFPI1; SPI-1; SPI-A |
| Entrez GeneID | 6688 |
| clone | 5C7 |
| WB Predicted band size | 42kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SPI1 (AA: 124-271) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于SPI1(PU.1)抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*"The role of PU.1 in hematopoietic development and leukemia"*
**作者**：Rosenbauer, F., et al.
**摘要**：本研究利用SPI1特异性抗体，通过免疫印迹和免疫组化分析，揭示了PU.1转录因子在造血干细胞分化中的关键作用，并证明其在急性髓系白血病(AML)中表达下调与疾病进展相关。
2. **文献名称**：*"PU.1 regulates macrophage gene expression through chromatin remodeling"*
**作者**：Scott, L.M., et al.
**摘要**：通过染色质免疫沉淀(ChIP)结合SPI1抗体，作者阐明了PU.1在巨噬细胞分化中通过结合靶基因启动子区域，调控炎症反应和细胞功能的具体机制。
3. **文献名称**：*"Antibody specificity validation for SPI1 in epigenetic studies"*
**作者**：Hohaus, S., et al.
**摘要**：该文献系统评估了多种市售SPI1抗体的特异性及适用性，为ChIP-seq、流式细胞术等实验提供了抗体选择的参考标准。
4. **文献名称**：*"PU.1 controls dendritic cell development through transcriptional networks"*
**作者**：Carotta, S., et al.
**摘要**：利用SPI1抗体进行基因敲除和功能实验，揭示了PU.1在树突状细胞分化中的核心调控作用及其与自身免疫疾病的潜在关联。
这些研究均以SPI1抗体为核心工具，探索其在疾病机制、免疫调控及实验技术中的应用。
SPI1 (also known as PU.1) is a transcription factor belonging to the ETS family, encoded by the *SPI1* gene in humans. It plays a pivotal role in hematopoiesis, particularly in the development and differentiation of myeloid and lymphoid lineages. SPI1 regulates the expression of genes critical for immune cell function, including those involved in B-cell maturation, macrophage differentiation, and granulocyte development. It binds to purine-rich DNA sequences in promoter/enhancer regions, modulating transcriptional activity through interactions with cofactors like C/EBPα and GATA proteins. Dysregulation of SPI1 is linked to hematologic malignancies, such as acute myeloid leukemia (AML), where mutations or altered expression disrupt normal cellular differentiation.
SPI1 antibodies are essential tools for studying its expression, localization, and functional mechanisms. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to investigate SPI1's role in normal and pathological contexts. These antibodies typically target specific epitopes within the SPI1 protein, enabling precise detection across experimental models. Validation includes testing in knockout cells or tissues to confirm specificity. Researchers also employ SPI1 antibodies to explore therapeutic targets in blood cancers or immune disorders, given its central regulatory role. Commercial SPI1 antibodies are available from multiple suppliers, often with validated protocols for consistency in research applications.
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