Mouse Monoclonal INCENP Antibody

The INCENP (Inner Centromere Protein) antibody is a key tool for studying the chromosomal passenger complex (CPC), a critical regulator of mitosis and meiosis. INCENP, along with Aurora B kinase, Survivin, and Borealin, forms the CPC, which ensures proper chromosome segregation, spindle assembly, and cytokinesis. Antibodies targeting INCENP are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to visualize CPC localization and dynamics during cell division. These antibodies often recognize specific epitopes, such as the C-terminal region essential for Aurora B binding. Validated in knockout cell lines or siRNA-treated samples, they confirm specificity and aid in studying mitotic errors linked to cancer and developmental disorders. Researchers also use INCENP antibodies to explore CPC dysfunction in tumorigenesis, as CPC dysregulation is associated with chromosomal instability in cancers. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary, influencing experimental applications. By enabling precise tracking of CPC behavior, INCENP antibodies remain vital for unraveling cell cycle mechanisms and therapeutic targets in diseases driven by mitotic defects.