Mouse Monoclonal E2F1 Antibody

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

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     Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.    

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     Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red).    

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     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using E2F1 mouse mAb with DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using E2F1 mouse mAb with DAB staining.    

The E2F1 antibody is a crucial tool in molecular biology for studying the E2F1 transcription factor, a key regulator of cell cycle progression and apoptosis. E2F1 belongs to the E2F family of proteins, which control the transition from the G1 to S phase by regulating genes essential for DNA replication and cell division. E2F1 activity is tightly controlled through interactions with the retinoblastoma (Rb) protein; Rb binding inhibits E2F1 until phosphorylation of Rb during late G1 phase releases E2F1. enabling transcriptional activation of target genes such as cyclin E and CDK2. Beyond its role in cell cycle regulation, E2F1 is implicated in DNA damage responses, differentiation, and oncogenesis, acting as both an oncogene and tumor suppressor depending on cellular context. Dysregulation of E2F1 is linked to cancers, making it a focus of therapeutic research.

E2F1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect E2F1 expression, localization, and DNA-binding activity. They help elucidate E2F1’s role in disease mechanisms, drug responses, and cellular pathways. Validated antibodies ensure specificity, often confirmed via knockout cell lines or siRNA knockdown. Researchers rely on these antibodies to explore E2F1’s dual functions in proliferation and apoptosis, offering insights into cancer biology and potential treatment strategies.