| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NSP5 |
| Uniprot No | Q5M775 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1068aa |
| 氨基酸序列 | MRSAAKPWNPAIRAGGHGPDRVRPLPAASSGMKSSKSSTSLAFESRLSRLKRASSEDTLNKPGSTAASGVVRLKKTATAGAISELTESRLRSGTGAFTTTKRTGIPAPREFSVTVSRERSVPRGPSNPRKSVSSPTSSNTPTPTKHLRTPSTKPKQENEGGEKAALESQVRELLAEAKAKDSEINRLRSELKKYKEKRTLNAEGTDALGPNVDGTSVSPGDTEPMIRALEEKNKNFQKELSDLEEENRVLKEKLIYLEHSPNSEGAASHTGDSSCPTSITQESSFGSPTGNQMSSDIDEYKKNIHGNALRTSGSSSSDVTKASLSPDASDFEHITAETPSRPLSSTSNPFKSSKCSTAGSSPNSVSELSLASLTEKIQKMEENHHSTAEELQATLQELSDQQQMVQELTAENEKLVDEKTILETSFHQHRERAEQLSQENEKLMNLLQERVKNEEPTTQEGKIIELEQKCTGILEQGRFEREKLLNIQQQLTCSLRKVEEENQGALEMIKRLKEENEKLNEFLELERHNNNMMAKTLEECRVTLEGLKMENGSLKSHLQGEKQKATEASAVEQTAESCEVQEMLKVARAEKDLLELSCNELRQELLKANGEIKHVSSLLAKVEKDYSYLKEICDHQAEQLSRTSLKLQEKASESDAEIKDMKETIFELEDQVEQHRAVKLHNNQLISELESSVIKLEEQKSDLERQLKTLTKQMKEETEEWRRFQADLQTAVVVANDIKCEAQQELRTVKRKLLEEEEKNARLQKELGDVQGHGRVVTSRAAPPPVDEEPESSEVDAAGRWPGVCVSRTSPTPPESATTVKSLIKSFDLGRPGGAGQNISVHKTPRSPLSGIPVRTAPAAAVSPMQRHSTYSSVRPASRGVTQRLDLPDLPLSDILKGRTETLKPDPHLRKSPSLESLSRPPSLGFGDTRLLSASTRAWKPQSKLSVERKDPLAALAREYGGSKRNALLKWCQKKTQGYANIDITNFSSSWSDGLAFCALLHTYLPAHIPYQELNSQEKKRNLLLAFEAAESVGIKPSLELSEMLYTDRPDWQSVMQYVAQIYKYFET |
| 预测分子量 | 118,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NSP5重组蛋白的3篇示例文献(内容为虚构，仅供格式参考)：
---
1. **文献名称**：*Expression and enzymatic characterization of recombinant SARS-CoV-2 NSP5 protease*
**作者**：Zhang Y. et al.
**摘要**：本研究在大肠杆菌系统中成功表达并纯化了SARS-CoV-2 NSP5重组蛋白，优化了表达条件以提高可溶性。通过荧光底物实验证实其蛋白酶活性，并筛选了潜在抑制剂，为抗病毒药物开发提供了基础。
2. **文献名称**：*Crystal structure analysis of NSP5 main protease from human coronaviruses*
**作者**：Li X. et al.
**摘要**：利用X射线晶体学解析了NSP5重组蛋白的三维结构，揭示了其催化二聚体界面及底物结合域的关键残基，为理解冠状病毒复制机制及靶向药物设计提供了结构依据。
3. **文献名称**：*Functional interaction between NSP5 and host proteins in viral replication*
**作者**：Wang J. et al.
**摘要**：通过重组NSP5蛋白与宿主细胞蛋白的共沉淀实验，发现其与特定宿主因子结合并调控病毒RNA合成，阐明了NSP5在逃逸天然免疫中的潜在作用。
---
注：以上文献为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。
Nonstructural protein 5 (NSP5), also known as the main protease (Mpro) or 3C-like protease (3CLpro), is a key enzyme encoded by coronaviruses, including SARS-CoV-2. It plays a critical role in viral replication by cleaving the polyproteins translated from the viral RNA into functional nonstructural proteins essential for assembling the replication-transcription complex. Due to its conserved catalytic mechanism and absence of closely related human homologs, NSP5 has emerged as a prime target for antiviral drug development.
Recombinant NSP5 refers to the protein produced through genetic engineering in heterologous expression systems, such as *E. coli* or mammalian cell lines. This approach allows large-scale production of the protease for structural and functional studies. The recombinant protein retains enzymatic activity, enabling researchers to study its substrate specificity, inhibition kinetics, and interactions with potential therapeutics. Structural studies using X-ray crystallography and cryo-EM, often relying on recombinant NSP5. have revealed its dimeric conformation and substrate-binding pockets, guiding rational drug design.
During the COVID-19 pandemic, recombinant NSP5 became pivotal in screening and optimizing protease inhibitors, such as nirmatrelvir (in Paxlovid), which disrupt viral replication. Additionally, studies using recombinant NSP5 variants have explored mutations associated with emerging variants (e.g., Omicron), assessing their impact on protease activity and drug resistance. Beyond therapeutics, recombinant NSP5 is used to develop diagnostic tools and investigate host-virus interactions, such as its role in evading innate immunity. Its conserved nature across coronaviruses also supports broad-spectrum antiviral research, highlighting its significance in pandemic preparedness.
×
