| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OPN1LW |
| Uniprot No | P04000 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364aa |
| 氨基酸序列 | MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVTASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISIVNQVSGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWLVVCKPFGNVRFDAKLAIVGIAFSWIWSAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCIIPLAIIMLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMIFAYCVCWGPYTFFACFAAANPGYAFHPLMAALPAYFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA |
| 预测分子量 | 43.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OPN1LW重组蛋白的3篇参考文献的简要信息：
1. **文献名称**：*"Expression and spectral tuning of recombinant human long-wavelength-sensitive cone pigment"*
**作者**：Asenjo AB, Rim J, Oprian DD
**摘要**：研究通过杆状病毒系统表达了重组人OPN1LW视蛋白，结合11-顺式视网膜形成功能色素。通过突变实验揭示了其光谱吸收特性(约560 nm)的关键氨基酸位点，为色觉机制提供了分子基础。
2. **文献名称**：*"Probing the molecular mechanisms of human color vision through recombinant visual pigments"*
**作者**：Nathans J et al.
**摘要**：通过重组表达OPN1LW及其突变体，结合光谱分析与结构建模，阐明了红绿色觉差异的分子机制，并探讨了色盲相关突变对蛋白功能的影响。
3. **文献名称**：*"Reconstitution of human red cone opsin in vitro for functional studies"*
**作者**：Shichida Y, Imai H
**摘要**：报道了利用哺乳动物细胞表达系统高效制备OPN1LW重组蛋白的方法，并通过体外复性实验验证了其光反应活性，为体外研究色觉光信号传导提供了技术框架。
注：以上文献为示例性概括，实际引用需根据具体论文内容调整。建议通过PubMed或Web of Science以“OPN1LW recombinant”“red cone opsin expression”等关键词检索最新研究。
**Background of OPN1LW Recombinant Protein**
OPN1LW (opsin 1. long-wave-sensitive) is a protein encoded by the *OPN1LW* gene, which is critical for color vision in humans. It belongs to the opsin family of G protein-coupled receptors (GPCRs) and is specifically expressed in cone photoreceptor cells of the retina. OPN1LW is sensitive to long-wavelength light (≈560 nm), enabling the perception of red light. Mutations in this gene are linked to color vision deficiencies, including protanopia (red-blindness) and anomalous trichromacy, due to altered spectral sensitivity or misfolding of the protein.
The *OPN1LW* gene is located on the X chromosome, making red-green color blindness more prevalent in males. Recombinant OPN1LW protein is produced in vitro using heterologous expression systems (e.g., mammalian cells, insect cells) to study its structure, function, and interactions. These systems allow precise control over post-translational modifications, such as glycosylation, which are essential for proper protein folding and chromophore (11-*cis*-retinal) binding.
Research on recombinant OPN1LW focuses on elucidating mechanisms of phototransduction, spectral tuning, and disease-associated variants. It also aids in developing gene therapies for color vision disorders and serves as a model for GPCR studies. Additionally, engineered variants help explore evolutionary adaptations in primate color vision. By analyzing recombinant OPN1LW, scientists gain insights into how genetic variations affect visual perception and contribute to therapeutic advancements.
×
