| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SORBS2 |
| Uniprot No | O94875 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 863-1100aa |
| 氨基酸序列 | KEKLPAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVGIFPISYVEKLTPPEKAQPARPPPPAQPGEIGEAIAKYNFNADTNVELSLRKGDRVILLKRVDQNWYEGKIPGTNRQGIFPVSYVEVVKKNTKGAEDYPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALYNYTPRNEDELELRESDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL |
| 预测分子量 | 33.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于SORBS2重组蛋白的示例参考文献(注：以下文献为虚构示例，仅供格式参考)：
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1. **文献名称**: *"Recombinant SORBS2 Protein Regulates Actin Cytoskeleton Dynamics in Cardiomyocytes"*
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌系统表达并纯化了人源SORBS2重组蛋白，发现其通过结合α-actinin调控心肌细胞中肌动蛋白骨架的重排，揭示了SORBS2在心脏收缩功能中的潜在作用。
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2. **文献名称**: *"Structural and Functional Characterization of SORBS2 SH3 Domains"*
**作者**: Müller S, et al.
**摘要**: 通过X射线晶体学解析了SORBS2重组蛋白的SH3结构域三维结构，并证明其特异性结合含有脯氨酸基序的靶蛋白(如Ponsin)，为研究细胞黏附复合物的组装机制提供了依据。
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3. **文献名称**: *"SORBS2 Recombinant Protein Attenuates TGF-β Signaling in Fibrotic Diseases"*
**作者**: Kim H, et al.
**摘要**: 在肝星状细胞中，外源性添加SORBS2重组蛋白可竞争性抑制TGF-β/Smad3信号通路，减少胶原沉积，表明其在抗纤维化治疗中的潜在应用价值。
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4. **文献名称**: *"Development of a High-Yield SORBS2 Expression System in Mammalian Cells"*
**作者**: Wang Y, et al.
**摘要**: 优化了HEK293细胞中SORBS2重组蛋白的分泌表达条件，获得高纯度蛋白，并验证其与胰岛素受体底物(IRS1)的相互作用，为糖尿病相关研究提供工具蛋白。
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如需真实文献，建议通过PubMed或Web of Science搜索关键词“SORBS2 recombinant”或结合具体研究领域筛选。
**Background of SORBS2 Recombinant Protein**
SORBS2 (Sorbin and SH3 Domain-Containing Protein 2), also known as ArgBP2. is a member of the SORBS protein family characterized by conserved sorbin homology (SoHo) and SH3 domains. It functions as an adaptor protein involved in cell signaling, cytoskeletal organization, and cell adhesion. The gene encoding SORBS2 is located on human chromosome 4q35.1 and is expressed in various tissues, including muscle, heart, and brain.
Structurally, SORBS2 contains three C-terminal SH3 domains that mediate protein-protein interactions and a central SoHo domain linked to membrane-associated processes. It interacts with signaling molecules such as c-Abl, vinculin, and synaptojanin, playing roles in regulating actin dynamics, focal adhesion assembly, and cellular responses to mechanical stress. SORBS2 is also implicated in pathways related to insulin signaling, TGF-β signaling, and apoptosis.
Recombinant SORBS2 protein is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. Its production enables *in vitro* studies to dissect molecular interactions, signaling mechanisms, and structural features. Research highlights its dual roles in health and disease: while it supports cardiac and neuronal function, dysregulation of SORBS2 is linked to pathologies such as cardiomyopathy, cancer metastasis, and metabolic disorders. For instance, reduced SORBS2 expression correlates with poor prognosis in breast cancer and hepatocellular carcinoma.
Overall, SORBS2 recombinant protein serves as a critical tool for exploring its biological functions and therapeutic potential in targeting related diseases.
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