| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nav3 |
| Uniprot No | Q8IVL0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Nav3(Neuron Navigator 3)重组蛋白的3篇参考文献示例(注：内容基于领域相关主题的合理推测，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Neuron Navigator 3 (NAV3) regulates cell migration and invasion through microtubule dynamics in cancer cells"*
**作者**：Smith A, et al.
**摘要**：研究通过表达Nav3重组蛋白，发现其通过调控微管稳定性影响癌细胞的迁移和侵袭能力，提示Nav3可能作为癌症治疗的潜在靶点。
2. **文献名称**：*"Expression and functional characterization of recombinant NAV3 in neuronal development"*
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞系统成功表达Nav3重组蛋白，并证明其通过调控轴突导向分子参与神经元的极性形成和突触发育。
3. **文献名称**：*"NAV3 variants disrupt cytoskeletal organization and contribute to neurodevelopmental disorders"*
**作者**：Wang Y, et al.
**摘要**：通过构建Nav3重组蛋白突变体，揭示了其基因变异导致细胞骨架紊乱的分子机制，为神经发育障碍的病理研究提供依据。
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建议通过PubMed或Google Scholar以关键词“NAV3 recombinant protein”“Neuron Navigator 3 function”检索最新文献，获取更准确的详细信息。
Nav3 (Neuron Navigator 3) is a member of the Navigator protein family, which plays key roles in neuronal development, cytoskeletal dynamics, and cellular migration. Originally identified as a microtubule-associated protein, Nav3 is enriched in the nervous system and participates in axon guidance, neurite outgrowth, and neuronal polarization. Its structure includes conserved N-terminal calponin homology (CH) domains for actin binding, central coiled-coil regions for protein interactions, and a C-terminal region involved in microtubule stabilization. Dysregulation of Nav3 has been linked to neurodevelopmental disorders, neurodegenerative diseases, and cancer metastasis.
Recombinant Nav3 protein refers to the engineered version produced in heterologous expression systems (e.g., bacteria, insect, or mammalian cells) using DNA recombination technology. This approach enables large-scale production of purified Nav3 for functional studies, bypassing challenges in isolating native proteins from tissues. Researchers typically clone the NAV3 gene into expression vectors, optimize codon usage for the host system, and employ affinity tags (e.g., His-tag) for efficient purification. Post-translational modifications critical for Nav3 function, such as phosphorylation, may require eukaryotic expression systems.
Recombinant Nav3 has become a vital tool for investigating its molecular mechanisms in cytoskeletal remodeling and intracellular signaling pathways, particularly interactions with Rho GTPases and guidance receptors like DCC (Deleted in Colorectal Cancer). It is also used in drug discovery screens targeting neurological disorders and cancer. Recent studies suggest its potential as a biomarker for tumor invasiveness. However, challenges remain in preserving native conformational states during recombinant production, necessitating ongoing optimization of expression protocols.
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