| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Atp1a3 |
| Uniprot No | P13637 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1013aa |
| 氨基酸序列 | MGDKKDDKDSPKKNKGKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCV QGLTHSKAQEILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAIL CFLAYGIQAGTEDDPSGDNLYLGIVLAAVVIITGCFSYYQEAKSSKIMES FKNMVPQQALVIREGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAHG CKVDNSSLTGESEPQTRSPDCTHDNPLETRNITFFSTNCVEGTARGVVVA TGDRTVMGRIATLASGLEVGKTPIAIEIEHFIQLITGVAVFLGVSFFILS LILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKN LEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTS FDKSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCI ELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGA PERILDRCSTILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYL PEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAG IKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDA KACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGA IVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVT GVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIMANIPLPLGTITILCI DLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLISMAYGQIGMI QALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYEQ RKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFE ETALAAFLSYCPGMDVALRMYPLKPSWWFCAFPYSFLIFVYDEIRKLILR RNPGGWVEKETYY |
| 预测分子量 | 138 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Atp1a3重组蛋白的3-4条参考文献示例(注：文献为虚构示例，实际引用需检索真实数据库)：
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1. **标题**: "Expression and Purification of Functional Recombinant ATP1A3 in Insect Cells"
**作者**: Tanaka M, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统表达人源ATP1A3重组蛋白，并通过亲和层析和尺寸排阻色谱纯化，证实重组蛋白具有Na+/K+-ATP酶活性，为疾病相关突变的功能研究提供工具。
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2. **标题**: "Structural Insights into ATP1A3 Dynamics Using Recombinant Protein Cryo-EM"
**作者**: Chen X, et al.
**摘要**: 通过冷冻电镜解析重组ATP1A3蛋白在脂质环境中的高分辨率结构，揭示其离子结合位点构象变化，为突变导致神经系统疾病的机制提供结构基础。
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3. **标题**: "ATP1A3 Mutant Recombinant Proteins Exhibit Impaired Autophosphorylation in vitro"
**作者**: Rodriguez S, et al.
**摘要**: 在大肠杆菌中表达野生型和突变型ATP1A3重组蛋白，发现致病突变(如D801Y)显著降低蛋白自磷酸化能力，提示其与交替性偏瘫的相关性。
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4. **标题**: "High-Yield Production of ATP1A3 Extracellular Domain in Mammalian Cells for Antibody Development"
**作者**: Kim H, et al.
**摘要**: 在HEK293细胞中高效表达ATP1A3胞外域重组蛋白，用于筛选特异性单克隆抗体，为靶向治疗和诊断工具开发奠定基础。
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**提示**：实际研究中，建议通过PubMed或Google Scholar检索关键词“ATP1A3 recombinant protein expression/purification/function”以获取真实文献。
**Background of ATP1A3 Recombinant Protein**
The ATP1A3 gene encodes the α3 subunit of Na+/K+-ATPase, a critical transmembrane enzyme responsible for maintaining electrochemical gradients across cell membranes by actively transporting sodium (Na⁺) and potassium (K⁺) ions. This P-type ATPase is predominantly expressed in neurons, playing a vital role in neuronal excitability, synaptic transmission, and overall CNS homeostasis. Dysregulation of ATP1A3 is linked to severe neurological disorders, including alternating hemiplegia of childhood (AHC), rapid-onset dystonia-parkinsonism (RDP), and developmental/epileptic encephalopathies.
Recombinant ATP1A3 protein is engineered to study the structure-function relationships, disease mechanisms, and therapeutic targets. Produced via heterologous expression systems (e.g., mammalian or insect cells), the recombinant protein retains post-translational modifications essential for its enzymatic activity. Purification typically involves affinity chromatography, yielding high-purity protein for functional assays.
Research applications include enzymatic activity studies to assess ATP hydrolysis kinetics, ion transport assays, and screening for inhibitors/modulators. Additionally, it aids in structural biology (e.g., cryo-EM or X-ray crystallography) to resolve conformational states during the Na+/K+ transport cycle. Disease-associated mutations (e.g., D801N, E815K) are often analyzed using recombinant ATP1A3 to elucidate their pathogenic effects on protein stability, ion affinity, or cellular localization.
Overall, ATP1A3 recombinant protein serves as a vital tool for understanding neurological pathologies and advancing targeted therapies.
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