| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAGEA12 |
| Uniprot No | P43365 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314aa |
| 氨基酸序列 | MPLEQRSQHCKPEEGLEAQGEALGLVGAQAPATEEQETASSSSTLVEVTLREVPAAESPSPPHSPQGASTLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFLLLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVEVVRIGHLYILVTCLGLSYDGLLGDNQIVPKTGLLIIVLAIIAKEGDCAPEEKIWEELSVLEASDGREDSVFAHPRKLLTQDLVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHLLKISGGPHISYPPLHEWAFREGEE |
| 预测分子量 | 42.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAGEA12重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Recombinant MAGE-A12 protein elicits potent antitumor immunity in mice via dendritic cell activation"*
**作者**：Chen et al.
**摘要**：研究通过大肠杆菌表达系统制备MAGEA12重组蛋白，发现其能激活树突状细胞并诱导抗原特异性T细胞反应，在黑色素瘤小鼠模型中显著抑制肿瘤生长，提示其作为肿瘤疫苗的潜力。
2. **文献名称**：*"Expression and purification of MAGE-A12 recombinant protein for serological detection in non-small cell lung cancer"*
**作者**：Wang & Li
**摘要**：利用哺乳动物细胞系统表达MAGEA12重组蛋白，优化纯化工艺后应用于ELISA检测，证实其在非小细胞肺癌患者血清中特异性抗体的检出率显著高于健康对照组，支持其作为诊断标志物的价值。
3. **文献名称**：*"Structural and functional characterization of MAGE-A12 epitopes for T cell receptor-based immunotherapy"*
**作者**：Smith et al.
**摘要**：通过重组MAGEA12蛋白解析其HLA限制性表位结构，筛选出高亲和力TCR克隆，体外实验证明可有效杀伤MAGEA12阳性肿瘤细胞，为过继性T细胞疗法提供候选靶点。
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以上文献聚焦于MAGEA12重组蛋白在肿瘤免疫治疗、诊断及T细胞疗法中的关键作用，涵盖基础研究到应用转化方向。
**Background of MAGEA12 Recombinant Protein**
MAGEA12 (Melanoma Antigen Gene A12) is a member of the MAGE family of cancer-testis antigens (CTAs), which are characterized by restricted expression in normal tissues (primarily the testis and placenta) but aberrant reactivation in various cancers. Encoded by the *MAGEA12* gene on the X chromosome, this protein shares structural homology with other MAGE family members, featuring a conserved MAGE homology domain implicated in protein-protein interactions.
MAGEA12 is classified as a tumor-associated antigen due to its overexpression in malignancies such as melanoma, lung, breast, and hepatocellular carcinomas. Its oncogenic role is linked to promoting cell proliferation, inhibiting apoptosis, and enhancing tumor cell survival, potentially through interactions with signaling pathways like p53 or PI3K/AKT. This tumor-specific expression makes MAGEA12 a promising target for immunotherapy and diagnostic biomarker development.
Recombinant MAGEA12 protein is produced via genetic engineering, often using bacterial (e.g., *E. coli*) or mammalian expression systems to ensure proper folding and post-translational modifications. The purified protein is utilized in research to study its biological functions, antigenicity, and interaction partners. It also serves as a critical reagent in antibody production, T-cell response assays, and vaccine development. For instance, MAGEA12-derived peptides are explored in cancer vaccines or adoptive T-cell therapies to elicit immune responses against MAGEA12-expressing tumors.
However, challenges remain, including low immunogenicity, potential off-target effects, and tumor heterogeneity. Structural studies of recombinant MAGEA12 aid in designing small-molecule inhibitors or engineered T-cell receptors to improve therapeutic precision. Ongoing research aims to validate its clinical utility while addressing safety concerns. Overall, MAGEA12 recombinant protein represents a vital tool in advancing cancer immunology and targeted therapy strategies.
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