| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Eef1akmt2 |
| Uniprot No | Q5JPI9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-236aa |
| 氨基酸序列 | MSSGADGGGGAAVAARSDKGSPGEDGFVPSALGTREHWDAVYERELQTFREYGDTGEIWFGEESMNRLIRWMQKHKIPLDASVLDIGTGNGVFLVELAKFGFSNITGIDYSPSAIQLSGSIIEKEGLSNIKLKVEDFLNLSTQLSGFHICIDKGTFDAISLNPDNAIEKRKQYVKSLSRVLKVKGFFLITSCNWTKEELLNEFSEGFELLEELPTPKFSFGGRSGNSVAALVFQKM |
| 预测分子量 | 25,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EEF1AKMT2(METTL21B)重组蛋白的简要参考文献列表：
1. **"METTL21B is a mammalian protein methyltransferase that modifies the heat shock protein HSP70"**
*作者：Cloutier P, et al.*
摘要：研究鉴定了METTL21B作为甲基转移酶，可在体外重组表达后对HSP70家族蛋白进行特异性赖氨酸甲基化修饰，揭示了其在分子伴侣调控中的作用。
2. **"Structural insights into the substrate recognition mechanism of METTL21B"**
*作者：Liu S, et al.*
摘要：通过重组蛋白结晶解析了METTL21B的催化结构域，阐明了其与底物eEF1A的结合模式及甲基化位点选择机制。
3. **"Proteomic analysis reveals EEF1AKMT2 as a novel regulator of translation elongation"**
*作者：Jakobsson ME, et al.*
摘要：利用重组EEF1AKMT2进行体外甲基化实验，证明其通过甲基化eEF1A调控蛋白质合成速率，影响细胞增殖过程。
4. **"METTL21B overexpression promotes tumor metastasis via eEF1A methylation in hepatocellular carcinoma"**
*作者：Wang Y, et al.*
摘要：重组METTL21B蛋白功能实验表明，其介导的eEF1A甲基化可激活癌症转移相关通路，提示其作为肝癌治疗靶点的潜力。
注：上述文献信息为基于领域知识的模拟概括，实际引用请以具体论文内容为准。
**Background of EEF1AKMT2 Recombinant Protein**
EEF1AKMT2 (Eukaryotic Elongation Factor 1 Alpha Lysine Methyltransferase 2), also known as METTL10 or FAM86B, is a member of the methyltransferase superfamily that catalyzes post-translational methylation of eukaryotic elongation factor 1 alpha (eEF1A), a critical GTPase involved in protein synthesis. This enzyme specifically targets a conserved lysine residue (K316 in humans) within the GTP-binding domain of eEF1A, modifying it through the addition of a methyl group using S-adenosylmethionine (SAM) as a cofactor. Methylation of eEF1A is evolutionarily conserved and implicated in regulating translation fidelity, stress response, and cytoskeletal dynamics.
The EEF1AKMT2 recombinant protein is engineered for in vitro studies to dissect its enzymatic activity, substrate specificity, and structural features. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the purified protein retains functional domains, including the catalytic SET domain and SAM-binding motifs. Research highlights its role in modulating eEF1A function, potentially influencing cellular processes such as proliferation, differentiation, and viral replication. Dysregulation of EEF1AKMT2 has been loosely associated with cancers and neurodegenerative disorders, though mechanistic insights remain under investigation.
Recombinant EEF1AKMT2 is a vital tool for biochemical assays, inhibitor screening, and structural studies, aiding in the exploration of methylation-dependent pathways in health and disease. Its study bridges gaps in understanding how post-translational modifications fine-tune translation machinery and cellular adaptation.
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