| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APOLD1 |
| Uniprot No | Q96LR9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-279aa |
| 氨基酸序列 | MFRAPCHRLRARGTRKARAGAWRGCTFPCLGKGMERPAAREPHGPDALRRFQGLLLDRRGRLHGQVLRLREVARRLERLRRRSLVANVAGSSLSATGALAAIVGLSLSPVTLGTSLLVSAVGLGVATAGGAVTITSDLSLIFCNSRELRRVQEIAATCQDQMREILSCLEFFCRWQGCGDRQLLQCGRNASIALYNSVYFIVFFGSRGFLIPRRAEGDTKVSQAVLKAKIQKLAESLESCTGALDELSEQLESRVQLCTKSSRGHDLKISADQRAGLFF |
| 预测分子量 | 33.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与APOLD1重组蛋白相关的模拟参考文献(基于公开研究主题的合理推测，非真实文献)：
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1. **标题**: *APOLD1重组蛋白调控血管内皮屏障功能的机制研究*
**作者**: Zhang L, et al.
**摘要**: 本研究通过原核表达系统制备了人源APOLD1重组蛋白，发现其通过激活RhoA/ROCK信号通路增强血管内皮细胞间连接，揭示了APOLD1在血管稳定性中的潜在作用。
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2. **标题**: *APOLD1重组蛋白在动脉粥样硬化模型中的治疗潜力*
**作者**: Müller C, et al.
**摘要**: 利用哺乳动物细胞表达系统获得高纯度APOLD1重组蛋白，动物实验表明其可减少动脉斑块炎症反应并改善脂质代谢，提示其作为动脉粥样硬化治疗靶点的可能性。
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3. **标题**: *APOLD1重组蛋白的晶体结构解析与功能域分析*
**作者**: Kim S, Watanabe H.
**摘要**: 首次报道了APOLD1重组蛋白的X射线晶体结构，确定其N端结构域具有结合VEGF受体的能力，为开发靶向血管生成抑制剂提供了结构基础。
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4. **标题**: *APOLD1重组蛋白通过调节自噬减轻缺血再灌注损伤*
**作者**: Chen R, et al.
**摘要**: 研究显示，外源性APOLD1重组蛋白可通过激活AMPK/mTOR通路促进心肌细胞自噬，显著降低小鼠心脏缺血再灌注后的组织损伤。
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**注**：以上内容为基于APOLD1已知生物学功能(如血管生成、细胞凋亡调控)的合理模拟，实际文献需通过PubMed或Web of Science等数据库检索确认。
APOLD1 (Apolipoprotein L Domain-Containing Protein 1) is a secreted glycoprotein encoded by the APOLD1 gene, primarily expressed in vascular endothelial cells and smooth muscle cells. First identified in 2003. it plays a regulatory role in vascular function and hemodynamic responses. The protein contains an N-terminal apolipoprotein L-like domain and a C-terminal disordered region, suggesting involvement in lipid metabolism and cell signaling. APOLD1 is dynamically regulated by mechanical forces like shear stress, linking it to blood flow adaptation and vascular remodeling processes.
Recombinant APOLD1 protein is typically produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications, including glycosylation. Purification often involves affinity tags like His-tag for standardized isolation. Its primary research applications focus on elucidating vascular biology mechanisms, particularly endothelial barrier function, angiogenesis, and inflammatory responses. Studies suggest APOLD1 interacts with integrins and modulates VEGF signaling pathways, making it relevant to atherosclerosis, hypertension, and tumor vascularization research. Recent investigations also explore its potential as a biomarker for cardiovascular diseases and its role in endoplasmic reticulum stress responses. The recombinant protein serves as a critical tool for in vitro assays studying endothelial cell behavior under flow conditions and for developing therapeutic strategies targeting vascular pathologies.
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