| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZSCAN1 |
| Uniprot No | Q8NBB4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MLPRPKAPASPRRPQTPTPSEQDADPGPASPRDTEAQRLRFRQFQYHVASGPHLALGQLWTLCRQWLRPEARSKEQMLELLVLEQFLGALPSKMRTWVQSQGPRSCREAASLVEDLTQMCQQEVLVSLDSVEPQDWSFGEEEDGKSPRSQKEPSQASELILDAVAAAPALPEESEWLETTQLQQSLHTRAEAEAPRAPGLLGSRARLPLKPSIWDEPEDLLAGPSSDLRAEGTVISSPKGPSAQRISPRRRNRNTDQSGRHQPSLKHTKGGTQEAVAGISVVPRGPRGGRPFQCADCGMVFTWVTHFIEHQKTHREEGPFPCPECGKVFLHNSVLTEHGKIHLLEPPRKKAPRSKGPRESVPPRDGAQGPVAPRSPKRPFQCSVCGKAFPWMVHLIDHQKLHTAHGHM |
| 预测分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZSCAN1重组蛋白的模拟参考文献示例(请注意，以下内容为虚构，实际文献需通过学术数据库查询确认)：
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1. **文献名称**: *Recombinant ZSCAN1 Protein Enhances Pluripotency in Embryonic Stem Cells*
**作者**: Zhang Y, Chen L, Wang F
**摘要**: 本研究通过昆虫细胞表达系统成功制备了重组ZSCAN1蛋白，并证实其通过结合Oct4和Nanog基因启动子区域调控胚胎干细胞多能性，为干细胞重编程机制提供了新见解。
2. **文献名称**: *Crystallization and Functional Analysis of ZSCAN1 Recombinant Protein*
**作者**: Kim S, Tanaka M, Park JH
**摘要**: 首次报道ZSCAN1重组蛋白的晶体结构，揭示其锌指结构域与DNA相互作用的分子机制，并验证其在体外对靶基因的转录激活能力。
3. **文献名称**: *High-Yield Production of ZSCAN1 in E. coli for Drug Screening Applications*
**作者**: Gupta R, Liu X, Wei D
**摘要**: 开发了一种高效的大肠杆菌表达系统，用于大规模生产可溶性ZSCAN1重组蛋白，并应用于小分子抑制剂筛选，为癌症治疗提供潜在靶点。
4. **文献名称**: *ZSCAN1 Recombinant Protein Modulates Telomerase Activity in Cancer Cells*
**作者**: Martinez E, Silva R, Gomez P
**摘要**: 发现ZSCAN1重组蛋白通过调控端粒酶逆转录酶(TERT)表达抑制肿瘤细胞增殖，提示其在癌症靶向治疗中的应用潜力。
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**备注**：实际研究中，ZSCAN1相关文献可能较少，建议通过PubMed或Google Scholar以“ZSCAN1 recombinant protein”、“ZSCAN1 expression”等关键词检索最新成果。如需真实文献，可提供具体研究方向以便进一步协助。
ZSCAN1 (Zinc Finger and SCAN Domain Containing 1) is a member of the SCAN-zinc finger protein family, characterized by conserved SCAN domains and C2H2-type zinc finger motifs. These structural features suggest its potential role in DNA binding and transcriptional regulation. The SCAN domain mediates protein-protein interactions, particularly with other SCAN-containing proteins, forming hetero- or homodimers that influence chromatin remodeling and gene expression. The zinc finger motifs, commonly found in transcription factors, likely enable sequence-specific DNA binding, positioning ZSCAN1 as a regulator of target genes.
While the full functional scope of ZSCAN1 remains under investigation, preliminary studies link it to early embryonic development, stem cell pluripotency, and cellular differentiation. In embryonic stem cells, ZSCAN1 expression correlates with maintenance of undifferentiated states, potentially through epigenetic modulation or direct transcriptional activation of pluripotency-associated genes. Its dynamic expression patterns during embryogenesis further support developmental roles.
Recombinant ZSCAN1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), retains DNA-binding and oligomerization capabilities, making it valuable for *in vitro* studies. Researchers utilize it to map DNA-binding sites, characterize protein interaction networks, and screen for small molecules modulating its activity. In disease contexts, aberrant ZSCAN1 expression has been observed in certain cancers and neurological disorders, though its pathogenic mechanisms remain unclear. Recombinant versions enable structural studies (e.g., X-ray crystallography) to resolve its molecular architecture and guide functional hypotheses.
Current challenges include identifying definitive target genes and clarifying its regulatory hierarchy within transcriptional networks. As interest grows in zinc finger proteins as therapeutic targets, recombinant ZSCAN1 serves as a critical tool for dissecting its biological significance and translational potential.
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