| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PPO2 |
| Uniprot No | O42713 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-378aa |
| 氨基酸序列 | MSLIATVGPTGGVKNRLNIVDFVKNEKFFTLYVRSLELLQAKEQHDYSSFFQLAGIHGLPFTEWAKERPSMNLYKAGYCTHGQVLFPTWHRTYLSVLEQILQGAAIEVAKKFTSNQTDWVQAAQDLRQPYWDWGFELMPPDEVIKNEEVNITNYDGKKISVKNPILRYHFHPIDPSFKPYGDFATWRTTVRNPDRNRREDIPGLIKKMRLEEGQIREKTYNMLKFNDAWERFSNHGISDDQHANSLESVHDDIHVMVGYGKIEGHMDHPFFAAFDPIFWLHHTNVDRLLSLWKAINPDVWVTSGRNRDGTMGIAPNAQINSETPLEPFYQSGDKVWTSASLADTARLGYSYPDFDKLVGGTKELIRDAIDDLIDERYG |
| 预测分子量 | 51.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPO2重组蛋白的模拟参考文献示例(注：文献为虚构，仅用于示例)：
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1. **文献名称**: *Expression and Characterization of Recombinant PPO2 in Escherichia coli for Enzymatic Browning Studies*
**作者**: Zhang, Y., et al.
**摘要**: 本研究通过大肠杆菌表达系统成功克隆并表达了马铃薯来源的PPO2重组蛋白，优化了诱导条件(0.5 mM IPTG, 16°C)。纯化后的酶比活性为120 U/mg，可显著催化多酚底物氧化，为果蔬褐变机制研究提供了工具。
2. **文献名称**: *Structural Insights into the Catalytic Mechanism of Recombinant Mushroom PPO2*
**作者**: Smith, J., et al.
**摘要**: 通过X射线晶体学解析了蘑菇PPO2重组蛋白的3D结构(分辨率2.1 Å)，发现其活性中心的铜离子配位模式独特。定点突变实验证实His208和His394为关键催化残基，阐明了底物特异性机制。
3. **文献名称**: *Heterologous Production of PPO2 in Pichia pastoris and Its Application in Biosensor Development*
**作者**: Lee, H., et al.
**摘要**: 在毕赤酵母中高效分泌表达PPO2.产量达200 mg/L。该重组酶被用于构建酚类污染物检测的生物传感器，对邻苯二酚的检测限低至0.1 μM，具备环境监测潜力。
4. **文献名称**: *Functional Analysis of Human PPO2 Recombinant Protein in Melanoma Cell Lines*
**作者**: Garcia, R., et al.
**摘要**: 研究发现人源PPO2重组蛋白在黑色素瘤细胞中过表达，通过调控多巴醌代谢促进肿瘤侵袭。利用siRNA敲低PPO2后，细胞迁移能力下降50%，提示其作为治疗靶点的可能性。
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如需真实文献，建议通过PubMed或Web of Science检索关键词“PPO2 recombinant protein”或结合具体研究领域筛选。
**Background of PPO2 Recombinant Protein**
Protoporphyrinogen oxidase (PPO, EC 1.3.3.4) is a critical enzyme in the heme and chlorophyll biosynthesis pathways, catalyzing the oxidation of protoporphyrinogen IX to protoporphyrin IX. PPO exists in two isoforms in plants: PPO1 (localized in mitochondria) and PPO2 (targeted to plastids). PPO2. the focus here, plays a vital role in chlorophyll production and is a key target for herbicides like acifluorfen and oxadiazon, which inhibit its activity, leading to protoporphyrin IX accumulation and light-dependent oxidative damage in plants.
Recombinant PPO2 protein is engineered for research and industrial applications. It is typically produced using heterologous expression systems, such as *E. coli* or yeast, by cloning the PPO2 gene into expression vectors. This allows large-scale production of the enzyme for functional studies, herbicide screening, and structural analysis. Purification methods often involve affinity chromatography (e.g., His-tag systems) followed by biochemical characterization to confirm enzymatic activity.
Research on PPO2 has advanced herbicide development and resistance management. Mutations in PPO2 linked to herbicide resistance (e.g., Gly210 deletions) are studied using recombinant proteins to understand resistance mechanisms. Additionally, PPO2’s role in human porphyrias—rare metabolic disorders caused by heme pathway defects—has spurred medical interest. Recombinant PPO2 aids in drug discovery for these conditions.
In summary, recombinant PPO2 serves as a versatile tool for agricultural, biochemical, and biomedical research, bridging plant physiology, herbicide innovation, and human health studies.
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