| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LGR6 |
| Uniprot No | Q9HBX8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-567aa |
| 氨基酸序列 | APQPGPGPTACPAPCHCQEDGIMLSADCSELGLSAVPGDLDPLTAYLDLSMNNLTELQPGLFHHLRFLEELRLSGNHLSHIPGQAFSGLYSLKILMLQNNQLGGIPAEALWELPSLQSLRLDANLISLVPERSFEGLSSLRHLWLDDNALTEIPVRALNNLPALQAMTLALNRISHIPDYAFQNLTSLVVLHLHNNRIQHLGTHSFEGLHNLETLDLNYNKLQEFPVAIRTLGRLQELGFHNNNIKAIPEKAFMGNPLLQTIHFYDNPIQFVGRSAFQYLPKLHTLSLNGAMDIQEFPDLKGTTSLEILTLTRAGIRLLPSGMCQQLPRLRVLELSHNQIEELPSLHRCQKLEEIGLQHNRIWEIGADTFSQLSSLQALDLSWNAIRSIHPEAFSTLHSLVKLDLTDNQLTTLPLAGLGGLMHLKLKGNLALSQAFSKDSFPKLRILEVPYAYQCCPYGMCASFFKASGQWEAEDLHLDDEESSKRPLGLLARQAENHYDQDLDELQLEMEDSKPHPSVQCSPTPGPFKPCEYLFESWGIRLA |
| 预测分子量 | 61.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LGR6重组蛋白的3篇代表性文献摘要(虚构示例，仅供参考)：
1. **文献名称**：*LGR6重组蛋白在毛囊干细胞激活中的作用机制研究*
**作者**：Smith A, et al.
**摘要**：该研究通过体外表达LGR6重组蛋白，证实其作为Wnt信号通路的共受体，可增强β-catenin活性，促进毛囊干细胞的增殖与分化，为组织再生提供分子基础。
2. **文献名称**：*LGR6重组蛋白的晶体结构解析及其配体结合特性*
**作者**：Zhang Y, et al.
**摘要**：研究利用重组LGR6蛋白进行X射线晶体学分析，首次揭示其胞外结构域与R-spondin配体的相互作用模式，阐明了LGR6参与调控干细胞微环境的分子机制。
3. **文献名称**：*靶向LGR6重组蛋白的抗体抑制乳腺癌转移的实验研究*
**作者**：Lee C, et al.
**摘要**：通过构建LGR6重组蛋白筛选单克隆抗体，发现其可阻断肿瘤细胞上皮-间质转化(EMT)，显著降低小鼠模型中乳腺癌细胞的肺转移能力，提示潜在治疗价值。
(注：以上文献为示例性内容，实际引用请通过PubMed/Web of Science检索真实文献)
LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a member of the rhodopsin-like G protein-coupled receptor (GPCR) family, characterized by a large extracellular domain containing leucine-rich repeats (LRRs). These receptors are evolutionarily conserved and play critical roles in developmental signaling, tissue homeostasis, and stem cell regulation. LGR6. specifically, is recognized for its involvement in Wnt/β-catenin signaling pathways by acting as a high-affinity receptor for R-spondins (RSPOs), secreted proteins that potentiate Wnt signaling. This interaction stabilizes Wnt receptors, enhancing pathway activity essential for stem cell maintenance, tissue regeneration, and embryonic development.
In mammals, LGR6 is expressed in various tissues, including skin, hair follicles, mammary glands, and the skeletal system. Studies highlight its role in regulating adult stem cells, particularly in epidermal and hair follicle niches, where it contributes to tissue repair and regeneration. For instance, LGR6+ stem cells in the skin have been shown to differentiate into multiple cell lineages, aiding wound healing and hair follicle cycling.
Recombinant LGR6 protein is engineered for in vitro and in vivo studies to dissect its molecular functions. Produced via heterologous expression systems (e.g., mammalian or insect cells), it retains the native structure required for ligand binding and signaling. Researchers utilize purified LGR6 to investigate receptor-ligand interactions, downstream signaling mechanisms, and its crosstalk with other pathways like BMP or Notch. Additionally, recombinant LGR6 serves as a tool in drug discovery, enabling screening for agonists/antagonists with therapeutic potential in regenerative medicine, cancer (due to its dual roles in tumor suppression/promotion), or degenerative diseases. Its study remains pivotal for understanding stem cell biology and developing targeted therapies.
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