| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR84 |
| Uniprot No | Q9NQS5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-26aa |
| 氨基酸序列 | MWNSSDANFSCYHESVLGYRYVAVSW |
| 预测分子量 | 31.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR84重组蛋白的3篇代表性文献示例(注：以下文献信息为示例性内容，实际引用需核实具体来源)：
1. **文献名称**：*Structural insights into ligand recognition and activation of the medium-chain fatty acid-sensing receptor GPR84*
**作者**：Wang Y. et al.
**摘要**：通过冷冻电镜解析GPR84与中链脂肪酸结合的三维结构，揭示了其配体结合口袋特征及激活下游信号通路的分子机制。
2. **文献名称**：*GPR84 is a pro-inflammatory receptor that enhances macrophage responses to lipopolysaccharide*
**作者**：Suzuki M. et al.
**摘要**：研究证明重组GPR84蛋白在巨噬细胞中高表达，通过增强TLR4信号通路促进炎症因子释放，提示其在炎症性疾病中的潜在调控作用。
3. **文献名称**：*Development of a novel radioligand binding assay for human GPR84 using recombinant protein expression*
**作者**：Zhang L. et al.
**摘要**：报道了一种基于HEK293细胞表达系统制备重组GPR84蛋白的方法，并建立放射性配体结合实验平台，用于高通量筛选GPR84拮抗剂。
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如需具体文献，建议通过PubMed或Web of Science以"GPR84 recombinant protein"为关键词检索近年研究，重点关注以下方向：
- 受体结构与功能研究
- 炎症/代谢疾病相关机制
- 药物筛选平台开发
- 中链脂肪酸(如癸酸)的配体作用机制
GPR84 is a class A G protein-coupled receptor (GPCR) primarily expressed in immune cells, including macrophages, neutrophils, and microglia. It was initially identified as an orphan receptor but was later deorphanized upon discovering its activation by medium-chain free fatty acids (MCFAs) with 9–14 carbon chains, such as lauric acid (C12:0). GPR84 plays a role in pro-inflammatory responses, linking metabolic changes to immune regulation. It is upregulated during tissue injury, infection, or metabolic stress, suggesting involvement in inflammation, fibrosis, and metabolic disorders. Signaling through GPR84 activates Gαi/o proteins, leading to reduced cAMP levels and modulation of pathways like MAPK and NF-κB, which drive cytokine production and immune cell migration.
Recombinant GPR84 proteins are engineered to study its structure, ligand interactions, and signaling mechanisms. These proteins are typically produced in heterologous expression systems (e.g., HEK293. insect, or bacterial cells) and often include tags (e.g., His, FLAG) for purification and detection. Recombinant forms may feature mutations to stabilize active/inactive conformations, enabling structural studies (e.g., X-ray crystallography, cryo-EM) or high-throughput drug screening. They are critical tools for identifying synthetic agonists/antagonists, as GPR84 is a therapeutic target for inflammatory diseases (e.g., colitis, pulmonary fibrosis), cancer, and metabolic syndrome. Recent studies also explore its role in neurodegenerative diseases, where neuroinflammation mediated by GPR84 may influence progression. However, its dual pro- and anti-inflammatory roles under different conditions require further investigation using recombinant systems to dissect context-dependent signaling.
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