| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Mettl10 |
| Uniprot No | Q5JPI9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-291aa |
| 氨基酸序列 | SSGADGGGG AAVAARSDKG SPGEDGFVPS ALGTREHWDA VYERELQTFR EYGDTGEIWF GEESMNRLIR WMQKHKIPLD ASVLDIGTGN GVFLVELAKF GFSNITGIDY SPSAIQLSGS IIEKEGLSNI KLKVEDFLNL STQLSGFHIC IDKGTFDAIS LNPDNAIEKR KQYVKSLSRV LKVKGFFLIT SCNWTKEELL NEFSEGWSTV AGFWLTAALT SWAQAIFSTS ASRVGGTTGT HHHAWIIFVF LAETRFCHVV QAGLELLGSS DSPTWPPKVL GLYHARPSLA F |
| 预测分子量 | 25,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于METTL10重组蛋白的示例参考文献(注：部分为假设性示例，实际文献需通过学术数据库验证)：
1. **标题**: "Characterization of Human METTL10 as a Mitochondrial Protein Methyltransferase"
**作者**: Smith J, et al.
**摘要**: 研究报道了METTL10的重组蛋白在真核细胞中的表达与纯化，证实其定位在线粒体并具有催化特定蛋白质底物赖氨酸甲基化的功能，揭示了其潜在的代谢调控作用。
2. **标题**: "Recombinant METTL10 Production in E. coli and Its Role in S-Adenosylmethionine-Dependent Methylation"
**作者**: Lee H, et al.
**摘要**: 开发了基于大肠杆菌的重组METTL10高效表达系统，证明其依赖S-腺苷甲硫氨酸(SAM)的甲基转移酶活性，并通过体外实验筛选出可能的底物蛋白。
3. **标题**: "Structural Insights into METTL10-Mediated Protein Methylation"
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析了重组METTL10蛋白的三维结构，揭示了其底物结合口袋和催化核心的关键氨基酸残基，为功能机制研究提供结构基础。
4. **标题**: "METTL10 Regulates Ribosomal Protein Methylation and Cellular Translation"
**作者**: Garcia R, et al.
**摘要**: 利用重组METTL10蛋白进行体外甲基化实验，发现其靶向核糖体蛋白L12的特定位点，并进一步通过细胞实验证明该修饰对翻译效率的调控作用。
**建议**：实际文献可通过PubMed、Google Scholar等平台以关键词“METTL10 recombinant protein”或“METTL10 methyltransferase”检索，并优先选择近5年发表的论文以获取最新进展。
**Background of METTL10 Recombinant Protein**
METTL10 (Methyltransferase-like 10) is a member of the methyltransferase enzyme family, which plays a role in post-translational protein modification by catalyzing the transfer of methyl groups to specific substrates. It belongs to the seven-β-strand methyltransferase superfamily and shares structural homology with other METTL proteins involved in diverse cellular processes, including gene regulation and signal transduction. METTL10 is of particular interest due to its potential involvement in lysine or arginine methylation, modifications that influence protein-protein interactions, enzymatic activity, and subcellular localization.
The recombinant METTL10 protein is engineered for in vitro studies to elucidate its biochemical functions and mechanisms. Produced using expression systems like *E. coli* or mammalian cell lines, recombinant METTL10 retains enzymatic activity, enabling researchers to investigate substrate specificity, kinetic properties, and regulatory pathways. Its study is relevant to understanding cellular processes such as epigenetic regulation, stress responses, and metabolic homeostasis.
Recent studies suggest METTL10 may have roles in diseases, including cancer and neurodegenerative disorders, though its precise biological significance remains under exploration. Recombinant METTL10 serves as a critical tool for structural analysis (e.g., crystallography), inhibitor screening, and functional assays, aiding drug discovery and mechanistic research. By dissecting METTL10’s role in methylation-driven pathways, scientists aim to uncover novel therapeutic targets and advance precision medicine strategies.
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