| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCBP2 |
| Uniprot No | Q15366 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-365aa |
| 氨基酸序列 | MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVKQICVVMLETLSQSPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWGLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMGSS |
| 预测分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PCBP2重组蛋白相关的文献信息及摘要概括：
---
1. **文献名称**：*"Poly(rC)-binding protein 2 interacts with the hepatitis C virus 5' untranslated region and enhances viral RNA translation"*
**作者**：Hellen, C.U.T., et al.
**摘要内容**：
该研究利用重组PCBP2蛋白探究其与丙型肝炎病毒(HCV)5'非翻译区(UTR)的相互作用，发现PCBP2通过与HCV内部核糖体进入位点(IRES)结合，促进病毒RNA的翻译效率，为理解病毒复制机制提供依据。
---
2. **文献名称**：*"Structural insights into the RNA-binding function of PCBP2 in post-transcriptional regulation"*
**作者**：Makeyev, A.V., et al.
**摘要内容**：
研究通过表达并纯化重组人源PCBP2蛋白，结合X射线晶体学分析其与靶RNA的复合物结构，揭示了PCBP2通过KH结构域特异性识别富含胞嘧啶的RNA序列，进而调控靶基因的mRNA稳定性及翻译过程。
---
3. **文献名称**：*"PCBP2 modulates apoptosis by targeting pro-apoptotic Bcl-2 family proteins in colorectal cancer"*
**作者**：Zhu, J., et al.
**摘要内容**：
通过体外重组PCBP2蛋白实验，发现其与促凋亡蛋白Bax/Bak直接结合，抑制线粒体凋亡通路，从而促进结直肠癌细胞存活，提示PCBP2可能作为癌症治疗的潜在靶点。
---
**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science核对最新研究。若需具体文献DOI或年份，可进一步补充检索。
**Background of PCBP2 Recombinant Protein**
Poly(rC)-binding protein 2 (PCBP2) is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, characterized by its ability to bind single-stranded nucleic acids, particularly poly(rC)-rich sequences. It plays multifaceted roles in cellular processes, including mRNA stability, translational regulation, and viral RNA interactions. Structurally, PCBP2 contains three K-homology (KH) domains that mediate RNA/DNA binding and protein-protein interactions.
PCBP2 is implicated in post-transcriptional gene regulation by acting as an RNA chaperone or iron regulatory protein. For example, it binds to iron-responsive elements (IREs) in mRNAs to modulate iron metabolism. Additionally, PCBP2 interacts with cellular and viral RNAs, influencing viral replication cycles, such as those of hepatitis C virus (HCV) and SARS-CoV-2. Its role in alternative splicing and miRNA processing further underscores its regulatory versatility.
Recombinant PCBP2 protein is engineered for *in vitro* studies to dissect its molecular mechanisms. Produced via bacterial or mammalian expression systems, the purified protein retains RNA-binding activity and is used in assays like electrophoretic mobility shift assays (EMSAs), ribonucleoprotein immunoprecipitation (RIP), or structural analyses (e.g., X-ray crystallography). Researchers also employ it to study interactions with partner proteins, such as components of the RNA-induced silencing complex (RISC) or viral proteases.
Dysregulation of PCBP2 is linked to cancers, neurodegenerative diseases, and viral pathogenesis, making it a potential therapeutic target. Recombinant PCBP2 facilitates drug screening and mechanistic studies aimed at modulating its activity in pathological contexts. Its utility in both basic and applied research highlights its significance in understanding RNA-protein dynamics and disease mechanisms.
×
