| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Spock2 |
| Uniprot No | Q92563 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-424aa |
| 氨基酸序列 | EGDAKGLK EGETPGNFME DEQWLSSISQ YSGKIKHWNR FRDEVEDDYI KSWEDNQQGD EALDTTKDPC QKVKCSRHKV CIAQGYQRAM CISRKKLEHR IKQPTVKLHG NKDSICKPCH MAQLASVCGS DGHTYSSVCK LEQQACLSSK QLAVRCEGPC PCPTEQAATS TADGKPETCT GQDLADLGDR LRDWFQLLHE NSKQNGSASS VAGPASGLDK SLGASCKDSI GWMFSKLDTS ADLFLDQTEL AAINLDKYEV CIRPFFNSCD TYKDGRVSTA EWCFCFWREK PPCLAELERI QIQEAAKKKP GIFIPSCDED GYYRKMQCDQ SSGDCWCVDQ LGLELTGTRT HGSPDCDDIV GFSGDFGSGV GWEDEEEKET EEAGEEAEEE EGEAGEADDG GYIW |
| 预测分子量 | 46,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的3篇关于SPOCK2重组蛋白的参考文献示例(实际文献请通过PubMed/Web of Science等数据库检索)：
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1. **标题**: *SPOCK2 promotes tumor progression via extracellular matrix remodeling in colorectal cancer*
**作者**: Li X, et al.
**摘要**: 本研究揭示了SPOCK2重组蛋白通过调控金属蛋白酶活性促进结直肠癌细胞外基质重塑，进而增强肿瘤细胞的迁移和侵袭能力。基因敲除实验表明SPOCK2通过Wnt/β-catenin通路影响肿瘤转移。
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2. **标题**: *Recombinant SPOCK2 protein inhibits neuronal differentiation through FGF signaling modulation*
**作者**: Tanaka K, et al.
**摘要**: 报道了重组SPOCK2蛋白在斑马鱼胚胎发育中的功能。实验证明SPOCK2通过结合成纤维细胞生长因子(FGF)抑制神经嵴细胞分化，为理解神经系统发育障碍提供了新机制。
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3. **标题**: *High-yield expression and purification of bioactive recombinant human SPOCK2 in E. coli*
**作者**: Zhang Y, et al.
**摘要**: 开发了一种基于大肠杆菌表达系统的SPOCK2重组蛋白高效制备方法，通过优化密码子和纯化流程获得高纯度蛋白。经圆二色谱验证其正确折叠，细胞实验证实其生物活性。
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**备注**：以上文献信息为学术示例，具体研究请以实际检索结果为准。建议使用关键词"SPOCK2 recombinant"或"SPOCK2 protein"在PubMed等平台获取最新文献。部分重要研究可能发表于《Matrix Biology》《Journal of Biological Chemistry》等期刊。
**Background of SPOCK2 Recombinant Protein**
SPOCK2. also known as *Sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2*, is a member of the SPOCK family, a group of extracellular matrix (ECM)-associated proteoglycans. Structurally, SPOCK2 contains conserved domains, including a follistatin-like domain, a calcium-binding EGF-like domain, and a Kazal-type serine protease inhibitor motif. These domains suggest its involvement in modulating cell-matrix interactions, protease activity, and signaling pathways. SPOCK2 is broadly expressed in tissues such as the brain, kidney, and immune cells, with emerging roles in development, tissue homeostasis, and disease.
Functionally, SPOCK2 regulates ECM remodeling by interacting with matrix metalloproteinases (MMPs) and integrins, influencing cell adhesion, migration, and differentiation. Studies link SPOCK2 to neurodevelopment, where it may guide neuronal growth and synaptic plasticity. In oncology, SPOCK2 is implicated in tumor progression, acting as either an oncogene or tumor suppressor depending on context. For instance, elevated SPOCK2 correlates with poor prognosis in gliomas, while its downregulation is observed in colorectal cancer.
The recombinant SPOCK2 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells*) to produce high-purity, functional protein for research. This tool enables mechanistic studies, such as elucidating SPOCK2’s role in ECM dynamics, immune regulation, or cancer metastasis. Additionally, it serves as an antigen for antibody development or a candidate for therapeutic targeting in diseases like neurodegeneration or fibrosis.
Despite progress, SPOCK2’s full biological spectrum remains under investigation. Its dual roles in health and disease highlight its potential as a biomarker or therapeutic target, driving interest in recombinant SPOCK2 for translational research.
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