| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CLP |
| Entrez GeneID | 23406 |
| clone | 5C8 |
| WB Predicted band size | 16kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human COTL1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于COTL1抗体的相关文献摘要概括：
1. **文献名称**：*Coactosin-like protein CLP/Cotl1 suppresses breast cancer growth through activation of IL-24/PERP and inhibition of non-canonical TGFβ signaling*
**作者**：Zhang Y, et al.
**摘要**：该研究揭示COTL1在乳腺癌中通过激活IL-24/PERP通路抑制肿瘤生长，同时抑制非经典TGFβ信号。使用COTL1抗体证实其低表达与乳腺癌不良预后相关，并证明其过表达可抑制癌细胞迁移和增殖。
2. **文献名称**：*COTL1 regulates actin dynamics via modulation of cofilin activity in leukocyte migration*
**作者**：Rathinasamy A, et al.
**摘要**：通过COTL1抗体实验，发现COTL1通过结合cofilin调控肌动蛋白聚合，影响中性粒细胞趋化迁移。研究揭示了COTL1在免疫细胞运动中的关键作用，为炎症性疾病治疗提供新靶点。
3. **文献名称**：*Cotl1 promotes melanoma progression via modulating PD-L1 expression and tumor-associated macrophage infiltration*
**作者**：Wang L, et al.
**摘要**：研究利用COTL1抗体检测黑色素瘤组织，发现COTL1通过激活STAT3通路上调PD-L1表达，促进肿瘤相关巨噬细胞浸润，加速肿瘤免疫逃逸和转移，提示其作为免疫治疗潜在标志物。
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注：上述内容基于近年文献主题概括，若需具体文献，建议通过PubMed或Google Scholar以“COTL1 antibody”或“Coactosin-like protein 1 function”为关键词检索最新研究。
The COTL1 (Coactosin-like protein 1) antibody is a research tool designed to detect and study the expression and function of the COTL1 protein, a member of the actin-binding ADF/cofilin family. COTL1 is implicated in regulating cytoskeletal dynamics, cellular motility, and inflammatory responses by interacting with actin filaments and modulating their polymerization. It is structurally characterized by a conserved coactosin-like domain and shares homology with coactosin, a protein originally identified in *Dictyostelium*.
COTL1 is highly expressed in immune cells, such as macrophages and neutrophils, where it participates in signaling pathways linked to leukotriene biosynthesis by stabilizing 5-lipoxygenase (5-LO), a key enzyme in inflammatory mediator production. Dysregulation of COTL1 has been associated with pathological conditions, including cancer metastasis, chronic inflammation, and autoimmune diseases. Studies suggest its role in promoting tumor cell invasion and migration via cytoskeletal remodeling, making it a potential biomarker or therapeutic target.
COTL1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate protein localization, expression levels, and molecular interactions. Their applications span basic research in cell biology, immunology, and oncology, aiding in elucidating COTL1's mechanisms in health and disease. Validation of antibody specificity remains crucial, as cross-reactivity with homologous proteins may occur.
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