| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | S6K; PS6K; S6K1; STK14A; p70-S6K; p70-alpha; p70(S6K)-alpha |
| Entrez GeneID | 6198 |
| clone | 5G9 |
| WB Predicted band size | 59kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RPS6KB1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于RPS6KB1抗体的参考文献示例(内容为模拟概括，非真实文献)：
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1. **文献名称**：*RPS6KB1 phosphorylation as a biomarker for mTOR pathway activation in breast cancer*
**作者**：Smith A et al.
**摘要**：研究使用RPS6KB1抗体(Western blot)检测乳腺癌组织中mTOR信号通路的活性，发现其磷酸化水平与肿瘤侵袭性相关。
2. **文献名称**：*Role of RPS6KB1 in regulating cellular senescence via antibody-mediated inhibition*
**作者**：Chen L et al.
**摘要**：通过RPS6KB1抗体阻断实验，揭示其在调控细胞衰老中的作用，表明靶向RPS6KB1可能延缓衰老相关疾病。
3. **文献名称**：*Validation of a novel RPS6KB1 monoclonal antibody for immunohistochemical applications*
**作者**：Park J et al.
**摘要**：报道一种高特异性RPS6KB1单克隆抗体的开发与验证，成功应用于前列腺癌组织样本的免疫组化检测。
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**注**：以上文献为示例，实际引用需查询PubMed或Google Scholar并核对原文。建议使用关键词“RPS6KB1 antibody”或“S6K1 antibody”结合研究领域(如癌症、代谢疾病)进行检索。
The RPS6KB1 antibody is a key tool in studying the ribosomal protein S6 kinase B1 (RPS6KB1), also known as p70S6K, a serine/threonine kinase central to the mTOR (mechanistic target of rapamycin) signaling pathway. This kinase regulates critical cellular processes, including protein synthesis, cell growth, proliferation, and metabolism by phosphorylating downstream targets like the S6 ribosomal protein. Dysregulation of RPS6KB1 is implicated in cancer, diabetes, and aging-related diseases, making it a focal point in therapeutic research, particularly for mTOR-targeted therapies.
The antibody detects RPS6KB1 in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), enabling researchers to assess protein expression, localization, and activation status. Phospho-specific RPS6KB1 antibodies (e.g., targeting Thr389) are widely used to monitor its activation by mTORC1. a critical step in pathway signaling. These antibodies aid in studying cellular responses to growth factors, nutrients, and stress, as well as evaluating drug efficacy in preclinical models.
Commercial RPS6KB1 antibodies are available as monoclonal or polyclonal variants, with validation often performed in knockout cell lines or tissues to ensure specificity. Their application spans cancer research (e.g., breast, prostate cancers), metabolic studies, and neurodegeneration, reflecting the kinase's broad regulatory role. Proper controls and optimization remain essential due to potential cross-reactivity with homologous kinases like RPS6KB2.
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