| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ksdD |
| Uniprot No | A0R4S9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-569aa |
| 氨基酸序列 | MFYMTGQEYDVVVVGSGAAGMVAALTAAHQGLSTVVVEKAPHYGGSTARSGGGVWIPNNEILKRDGVKDTPDEARKYLHAIIGDVVPAEKIDTYLDRGPEMLSFVLKHSPLKLCWVPGYSDYYPETPGGKPTGRSVEPKPFDANKLGPDLKGLEPPYGKVPMNMVVMQQDYVRLNQLKRHPRGVLRSLKVGIRATWGKVSGKNLVGMGRALIAPLRIGLREAGVPVLLNTALTDLYVEDGRVLGIYVRDTTAGDDAEPRLIRARHGVILGSGGFEHNEQMRVKYQRAPITTEWTVGAVANTGDGIVAAEKLGAALELMEDAWWGPTVPLVGAPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGQYGQGPGPGENIPAWLIFDQQYRDRYIFAGLQPGQRIPSKWLESGVIVKADTLVELAEKTGLPADQFTSTIERFNGFARSGVDEDFHRGESAYDRYYGDPTNKPNPNLGEIKHGPFYAAKMVPGDLGTKGGIRTDVRGRALRDDDSVIEGLYAAGNVSSPVMGHTYPGPGGTIGPAMTFGYLAALDIAATAAASRKG |
| 预测分子量 | 68.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ksdD重组蛋白的3篇代表性文献摘要(基于模拟生成，非真实文献)：
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1. **文献名称**: *Cloning, expression, and characterization of ksdD gene encoding a thermostable ketosteroid dehydrogenase in Escherichia coli*
**作者**: Zhang Y, et al.
**摘要**: 本研究克隆了来自放线菌的ksdD基因，并在大肠杆菌中实现高效可溶性表达。重组蛋白经纯化后表现出对Δ1-类固醇底物的高脱氢活性，酶学性质分析显示其最适温度为60°C，具有良好的热稳定性，适用于工业级类固醇生物转化。
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2. **文献名称**: *Structural insights into the catalytic mechanism of ksdD 3-ketosteroid dehydrogenase through crystallographic analysis*
**作者**: Müller C, et al.
**摘要**: 通过X射线晶体学解析ksdD重组蛋白的3D结构(分辨率2.1Å)，揭示了其底物结合口袋的关键氨基酸残基。结合分子对接实验，阐明了该酶催化类固醇C1-C2脱氢的分子机制，为理性设计高活性突变体奠定基础。
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3. **文献名称**: *Enhancement of ksdD recombinant protein production using a novel auto-induction medium in Bacillus subtilis*
**作者**: Li H, et al.
**摘要**: 开发了一种枯草芽孢杆菌表达系统，通过优化启动子与培养基成分，使ksdD重组蛋白产量提高3.2倍。功能验证表明重组酶能高效转化植物甾醇为关键药物中间体，为规模化生产提供低成本方案。
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注：以上文献为模拟生成，实际研究中建议通过PubMed/Google Scholar以“ksdD recombinant protein”或“3-ketosteroid dehydrogenase heterologous expression”等关键词检索真实文献。
KsdD recombinant protein, derived from microbial sources such as *Mycobacterium* or *Rhodococcus* species, is a key enzyme in steroid metabolism. Functionally classified as a ketosteroid monooxygenase, KsdD catalyzes the degradation of sterols and steroid derivatives, specifically targeting the cleavage of the steroid ring structure. This enzyme plays a critical role in microbial steroid degradation pathways, enabling organisms to utilize sterols as carbon sources. Its activity is particularly associated with the oxidation of Δ1-dehydrogenation in steroids, a reaction pivotal in pharmaceutical and industrial processes for modifying steroid backbones to produce bioactive compounds like corticosteroids or contraceptives.
The recombinant form of KsdD is engineered via heterologous expression systems (e.g., *E. coli* or yeast) to enhance production efficiency and stability. Cloning the *ksdD* gene into expression vectors allows large-scale purification, overcoming limitations of low native expression in wild-type strains. Structural studies reveal that KsdD belongs to the flavin-dependent monooxygenase family, requiring FAD as a cofactor for catalytic activity. Its substrate flexibility and regioselectivity make it valuable in biotransformation applications, including the synthesis of high-value steroid intermediates and environmental bioremediation of steroid pollutants.
Research on KsdD has expanded due to its potential in green chemistry, reducing reliance on harsh chemical synthesis methods. Additionally, its role in pathogenicity mechanisms of certain bacteria (e.g., *Mycobacterium tuberculosis*) has spurred interest in targeting KsdD for antimicrobial drug development. Current challenges include optimizing thermostability and catalytic efficiency for industrial scalability. Overall, KsdD recombinant protein bridges biotechnological innovation with sustainable steroid production and therapeutic discovery.
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