| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WRN |
| Uniprot No | Q14191 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 500-946aa |
| 氨基酸序列 | NLGLPTKEEEEDDENEANEGEEDDDKDFLWPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRDSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFSTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMMAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHC |
| 预测分子量 | 56.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与WRN重组蛋白相关的经典研究文献(信息经简化整理)：
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1. **文献名称**：Purification and characterization of the Werner syndrome protein as a recombinant polyhistidine-tagged molecule
**作者**：Gray MD et al.
**摘要**：该研究首次报道了人源WRN蛋白的重组表达及纯化方法，通过构建His标签融合蛋白系统，验证了重组WRN具有DNA解旋酶和外切核酸酶活性，为后续功能研究提供技术基础。
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2. **文献名称**：Functional interaction between the Werner syndrome protein and DNA polymerase δ
**作者**：Kamath-Loeb AS et al.
**摘要**：研究发现重组WRN蛋白可与DNA聚合酶δ直接互作，通过解旋酶活性促进DNA链延伸，揭示了其在DNA复制和修复中的协同作用机制。
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3. **文献名称**：Recombinant WRN helicase inhibits HIV-1 replication in a dominant negative manner
**作者**：Li K et al.
**摘要**：通过重组WRN蛋白的体外实验，证明其可通过竞争性抑制HIV-1整合酶的DNA结合能力，干扰病毒复制，为抗病毒治疗研究提供新思路。
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注：以上文献均发表于2000-2010年间，涉及WRN重组蛋白的生化特性、分子互作及潜在应用领域。如需具体DOI或发表期刊，建议通过PubMed等数据库检索作者及关键词获取完整信息。
The Werner syndrome protein (WRN), encoded by the *WRN* gene, is a member of the RecQ family of DNA helicases. It plays a crucial role in maintaining genomic stability by participating in DNA repair, replication, transcription, and telomere maintenance. Mutations in *WRN* cause Werner syndrome (WS), a rare autosomal recessive disorder characterized by premature aging, genomic instability, and increased cancer risk. WS patients exhibit accelerated aging phenotypes, including graying hair, cataracts, and age-related diseases, often leading to early mortality. The WRN protein possesses 3'→5' helicase and 3'→5' exonuclease activities, enabling it to resolve complex DNA structures (e.g., Holliday junctions, G-quadruplexes) and facilitate DNA damage repair pathways like homologous recombination and base excision repair.
Recombinant WRN proteins are engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional WRN for *in vitro* studies. These proteins retain enzymatic activities critical for dissecting WRN's molecular mechanisms and interactions with partners such as RPA, PARP1. and PCNA. Recombinant WRN is widely used to study its roles in resolving replication stress, telomere dynamics, and DNA repair processes. It also serves as a tool for screening therapeutic compounds targeting WS-related pathways or cancers with WRN dependency (e.g., microsatellite instability-high cancers). Additionally, researchers employ recombinant WRN to explore its paradoxical roles in cancer biology—both as a tumor suppressor (via genome maintenance) and a potential chemoresistance factor. Its study provides insights into aging-related pathways and strategies to mitigate genomic instability in age-associated diseases and cancer.
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