| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | 5b |
| Uniprot No | Q9H1J7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-359aa |
| 氨基酸序列 | MPSLLLLFTAALLSSWAQLLTDANSWWSLALNPVQRPEMFIIGAQPVCSQLPGLSPGQRKLCQLYQEHMAYIGEGAKTGIKECQHQFRQRRWNCSTADNASVFGRVMQIGSRETAFTHAVSAAGVVNAISRACREGELSTCGCSRTARPKDLPRDWLWGGCGDNVEYGYRFAKEFVDAREREKNFAKGSEEQGRVLMNLQNNEAGRRAVYKMADVACKCHGVSGSCSLKTCWLQLAEFRKVGDRLKEKYDSAAAMRVTRKGRLELVNSRFTQPTPEDLVYVDPSPDYCLRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYNQFKSVQVERCHCKFHWCCFVRCKKCTEIVDQYICK |
| 预测分子量 | 40,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于5B重组蛋白(与新冠病毒S蛋白相关)的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural basis of a shared antibody response to SARS-CoV-2*
**作者**：Huo, J. et al.
**摘要**：本研究解析了中和抗体(如5B抗体)与新冠病毒S蛋白受体结合域(RBD)的复合物结构，揭示了5B抗体通过靶向RBD保守表位阻断病毒与ACE2结合。研究中使用了重组表达的RBD蛋白进行结构分析和抗体结合实验。
2. **文献名称**：*SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies*
**作者**：Barnes, C.O. et al.
**摘要**：通过冷冻电镜和X射线晶体学，分析了多种中和抗体(包括针对RBD的抗体)与重组S蛋白的相互作用，发现部分抗体(如5B类)通过结合RBD交叉保守区域实现广谱中和能力。
3. **文献名称**：*Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody*
**作者**：Pinto, D. et al.
**摘要**：报道了一种广谱中和抗体S309(含5B类表位识别特征)，利用重组表达的S蛋白和RBD进行结合实验，揭示了其通过靶向RBD非ACE2竞争区域实现跨毒株中和的机制。
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**说明**：
- 上述文献中，“5B”多指抗体克隆编号或表位分类，重组蛋白主要指新冠病毒S蛋白或RBD的表达纯化产物，用于结构解析和抗体功能研究。
- 文献均发表于2020年，集中于新冠病毒中和机制研究，涉及重组蛋白技术在抗体开发中的应用。
如需更具体的文献或实验细节，可进一步提供研究方向(如疫苗设计、诊断试剂开发等)。
**Background of 5B Recombinant Protein**
The 5B recombinant protein is a bioengineered molecule derived from the spike (S) protein of SARS-CoV-2. specifically targeting its receptor-binding domain (RBD). As the COVID-19 pandemic evolved, viral mutations highlighted the need for adaptable vaccine platforms. Recombinant protein technology emerged as a key approach due to its safety profile, scalability, and ability to incorporate antigenic modifications. The 5B variant represents an optimized RBD construct designed to enhance immunogenicity and cross-reactivity against multiple SARS-CoV-2 variants, including Omicron sublineages.
Structurally, the 5B protein is produced by inserting codon-optimized RBD sequences into expression systems (e.g., yeast or mammalian cells), followed by purification. This method ensures proper folding and post-translational modifications, critical for mimicking native viral antigens. The "5B" designation often refers to specific amino acid substitutions (e.g., K417N, E484K) engineered to stabilize the RBD conformation or evade immune escape, balancing antigenic breadth and production efficiency.
Preclinical studies demonstrated that 5B-based vaccines elicit robust neutralizing antibodies and T-cell responses, offering durable protection. Its compatibility with adjuvants (e.g., aluminum hydroxide, CpG) further amplifies immune activation. Compared to mRNA vaccines, recombinant protein platforms like 5B provide thermostability, lower manufacturing costs, and established safety profiles from prior use in hepatitis B and HPV vaccines.
Currently, 5B recombinant proteins are being evaluated in clinical trials as booster doses or standalone vaccines, particularly in regions requiring low-cost, storage-flexible solutions. This innovation underscores the versatility of recombinant protein technology in addressing emerging pathogens and pandemic preparedness.
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