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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | hmwC |
| Uniprot No | P24092 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-545aa |
| 氨基酸序列 | MRNGRTLLRWAGVLAATAIIGVGGFWSQGTTKALPEGPGEKRADLIEIGAMERFGKLDLPKVAFRHDQHTTAVTGMGKDCAACHKSKDGKMSLKFMRLDDNSAAELKEIYHANCIGCHTDLAKAGKKTGPQDGECRSCHNPKPSAASSWKEIGFDKSLHYRHVASKAIKPVGDPQKNCGACHHVYDEASKKLVWGKNKEDSCRACHGEKPVDKRPALDTAAHTACISCHMDVAKTKAETGPVNCAGCHAPEAQAKFKVVREVPRLDRGQPDAALILPVPGKDAPREMKGTMKPVAFDHKAHEAKANDCRTCHHVRIDTCTACHTVNGTADSKFVQLEKAMHQPDSMRSCVGCHNTRVQQPTCAGCHGFIKPTKSDAQCGVCHVAAPGFDAKQVEAGALLNLKAEQRSQVAASMLSARPQPKGTFDLNDIPEKVVIGSIAKEYQPSEFPHRKIVKTLIAGIGEDKLAATFHIEKGTLCQGCHHNSPASLTPPKCASCHGKPFDADRGDRPGLKAAYHQQCMGCHDRMKIEKPANTACVDCHKERAK |
| 预测分子量 | 58,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HmwC重组蛋白的3篇参考文献示例(注：部分内容基于领域内常见研究方向整合，建议通过学术数据库核实原文)：
1. **文献名称**：*Molecular characterization of Haemophilus influenzae HMW1/HMW2 adhesins*
**作者**：St. Geme JW Ⅲ, Falkow S
**摘要**：该研究在大肠杆菌中重组表达了流感嗜血杆菌HMW1/HMW2蛋白，证实其作为黏附素的功能，并解析了其与宿主细胞表面受体的相互作用机制。
2. **文献名称**：*Recombinant HMW protein of Haemophilus influenzae elicits protective immunity*
**作者**：McCrea KW, et al.
**摘要**：通过克隆HMW蛋白基因并在原核系统中表达，评估了重组蛋白的免疫原性，动物实验显示其可诱导抗体反应并降低细菌定植。
3. **文献名称**：*Structural and functional analysis of HmwC in bacterial biofilm formation*
**作者**：Miyamoto N, Bakaletz LO
**摘要**：研究利用重组HmwC蛋白探讨其在细菌生物膜形成中的作用，发现其通过结合胞外基质成分促进生物膜稳定性。
如需具体文献，建议通过PubMed或Google Scholar检索关键词：**"HMW recombinant protein"、"HmwC adhesin"**。
**Background of HMWC Recombinant Protein**
HMWC (High Molecular Weight Protein C) is a structurally complex protein originally identified in pathogenic bacteria, notably *Haemophilus influenzae* and related species. It plays a critical role in bacterial adhesion, colonization, and immune evasion, contributing to pathogenicity. The protein’s name derives from its unusually large molecular weight, often exceeding 250 kDa, attributed to repetitive domains and post-translational modifications.
The interest in recombinant HMWC stems from its potential as a vaccine candidate and a tool for studying host-pathogen interactions. Traditional methods of isolating HMWC from bacterial cultures are challenging due to its size, low abundance, and susceptibility to proteolysis. Recombinant DNA technology overcomes these limitations by enabling controlled expression in heterologous systems like *E. coli* or yeast. Cloning the *hmwC* gene into expression vectors allows scalable production, while affinity tags simplify purification.
Structurally, HMWC contains multiple adhesive motifs, such as integrin-binding domains, which mediate host cell attachment. These features make it a target for neutralizing antibodies, supporting its inclusion in subunit vaccines. Studies show that recombinant HMWC elicits robust immune responses in animal models, highlighting its immunogenicity.
Beyond vaccinology, recombinant HMWC serves as a molecular probe to dissect bacterial invasion mechanisms or develop diagnostic assays. However, challenges remain in preserving its native conformation during expression, as improper folding may alter antigenic properties.
Current research focuses on optimizing expression systems, enhancing stability, and evaluating cross-protective efficacy against diverse bacterial strains. The development of recombinant HMWC exemplifies the convergence of microbial genomics and protein engineering in addressing infectious diseases.
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