Mouse Monoclonal TNNI2 Antibody

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

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     Immunofluorescence analysis of Hela cells using TNNI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

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     Flow cytometric analysis of NIH/3T3 cells using TNNI2 mouse mAb (green) and negative control (red).    

The TNNI2 antibody is a crucial tool for studying the fast-twitch skeletal muscle isoform of troponin I (TNNI2), a key regulatory protein in muscle contraction. TNNI2. encoded by the *TNNI2* gene, is predominantly expressed in fast skeletal muscle fibers and interacts with troponin C and T to regulate calcium-dependent actin-myosin interactions. Antibodies targeting TNNI2 are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant proteins, ensuring specificity through techniques such as ELISA or affinity purification. These antibodies are widely used in Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect TNNI2 expression in tissues or cell models, aiding research on muscle development, regeneration, and diseases like muscular dystrophy or myopathies.

In research, TNNI2 antibodies help distinguish fast-twitch from slow-twitch or cardiac muscle fibers, providing insights into muscle-specific pathologies. They also serve as biomarkers in studies on exercise physiology, sarcopenia, and certain cancers (e.g., rhabdomyosarcoma). However, cross-reactivity with other troponin isoforms or species variations (e.g., human vs. rodent) requires validation via knockout controls or sequence alignment. Commercial antibodies often specify applications and species reactivity, but batch variability may affect reproducibility. Alternative names, such as FS-TnI or TnI-fast, should be considered when searching for reagents. Overall, TNNI2 antibodies are indispensable for exploring skeletal muscle biology and related disorders.