| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ly6a |
| Uniprot No | P05533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-112aa |
| 氨基酸序列 | LECYQCYGVPFETSCPSITCPYPDGVCVTQEAAVIVDSQTRKVKNNLCLPICPPNIESMEILGTKVNVKTSCCQEDLCNVAVPNGG |
| 预测分子量 | 16.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ly6a(Sca-1)重组蛋白的3篇代表性文献摘要概览：
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1. **文献名称**：*Structural insights into Ly6a/Sca-1: Implications for stem cell surface marker function*
**作者**：Kim et al.
**摘要**：通过重组表达Ly6a蛋白并结合X射线晶体学技术，解析了其三维结构，揭示了其独特的GPI锚定蛋白折叠特征，并发现其可能的配体结合区域，为理解其在干细胞分选中的作用提供结构基础。
2. **文献名称**：*Ly6a recombinant protein enhances hematopoietic stem cell isolation in murine models*
**作者**：Garcia et al.
**摘要**：开发了高纯度重组Ly6a蛋白，验证其作为抗体替代物在流式分选中的效能，证明其可特异性结合小鼠造血干细胞，显著提升分选效率，为干细胞研究提供新工具。
3. **文献名称**：*Ly6a-mediated signaling in tumor immunity: Role of recombinant protein screening*
**作者**：Wang et al.
**摘要**：利用重组Ly6a蛋白进行体外实验，发现其通过调控T细胞表面受体互作影响免疫应答，提示Ly6a在肿瘤微环境中的潜在免疫调节功能，为癌症治疗提供新靶点。
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以上文献方向涵盖结构解析、技术应用及功能机制，可作为Ly6a重组蛋白研究的参考切入点。
Ly6a, also known as stem cell antigen-1 (Sca-1), is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the LY6/urokinase-type plasminogen activator receptor (uPAR) superfamily. It is widely recognized as a key marker for murine hematopoietic stem cells (HSCs) and progenitor cells, playing critical roles in cell adhesion, signaling, and immune regulation. Ly6a is encoded by the *Ly6a* gene and is structurally characterized by a conserved LU domain containing 8-10 cysteine residues that form disulfide bonds, stabilizing its three-fingered protein fold. This structure facilitates interactions with ligands or receptors in cellular microenvironments.
Recombinant Ly6a protein is engineered through genetic modification, typically expressed in heterologous systems like mammalian cells or *E. coli*, to ensure proper folding and post-translational modifications. Its production enables functional studies, such as investigating ligand-receptor interactions, stem cell maintenance mechanisms, and immune cell modulation. Ly6a has been implicated in regulating HSC self-renewal, T-cell activation, and cancer progression, where its overexpression in certain tumors correlates with metastatic potential. Researchers utilize recombinant Ly6a in flow cytometry, antibody development, and *in vitro* assays to dissect its biological roles. Despite its prominence in murine models, human orthologs of Ly6a remain elusive, though LY6 family members like LY6E or LY6K share structural homology. This underscores Ly6a’s importance as a model for understanding stem cell biology and immune responses in translational research.
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